巨噬细胞CD68、IL-6在子宫腺肌病组织中表达的研究

目的探讨巨噬细胞CD68、IL-6子宫腺肌病发病机制的作用。方法采用免疫组化法检测巨噬细胞CD68、IL-6在58例子宫腺肌病病灶及结合带组织中的表达,以同期54例子宫肌瘤、30例子宫腺肌病合并肌瘤、30例CINⅢ为对照。结果 (1)CD68、IL-6在子宫腺肌病病灶组织及结合带组织中的表达均高于其在子宫肌瘤及CINⅢ组织中的表达,差异有统计学意义(P<0.001);(2)CD68、IL-6在子宫腺肌病病灶组织的表达高于其在腺肌病结合带组织的表达(P<0.001);(3)CD68、IL-6在子宫肌瘤病灶组织、结合带组织及CINⅢ结合带组织中的表达差异无统计学意义(P>0.05);(4)子宫腺肌病合并子宫肌瘤组CD68、IL-6在腺肌病病灶组织中的表达均高于其在肌瘤病灶组织中的表达,差异有统计学意义(P<0.001);(5)Pearson相关分析显示:在子宫腺肌病病灶组织中CD68与IL-6的表达呈正相关(r=0.303,P=0.021)。结论巨噬细胞CD68与IL-6可能共同参与了子宫腺肌病的发生发展,在子宫腺肌病的发病机制中发挥着重要作用。

Study on the expression of CD68 and IL-6 in adenomyosis

Objective To explore the roles of macrophage and IL-6 in the pathogenesis of adenomyosis（AM）.Methods The CD68 is employed to mark macrophages, immunohistochemical staining were used to detect the expression of CD68 and IL-6 in ectopic endometrial and junction zone specimens in 58 cases of patients with adenomyosis, compared with 54 cases of patients with leiomyoma, 30 cases of patients with adenomyosis associated with leiomyoma, and 30 cases of patients with CINⅢ. Results The expression of CD68 and IL-6 in ectopic endometrial and junction zone specimens in AM group were significantly higher than that in leiomyoma group and CINⅢ group （P〈0.001）. The expression of CD68 and IL-6 in ectopic endometrial in AM were significantly higher than that in junction zone （P〈0.001）. The expression of CD68 and IL-6 didn＇t show significant difference between leiomyoma group and CINⅢ （P〉0.05）, and so between myoma tissue samples and junction zone in leiomyoma group （P〉0.05）. The expression of CD68 and IL-6 in adenomyoma tissues were significantly higher than that in myoma tissues in 30 cases of patients with adenomyosis associated with leiomyoma （P〈0.001）. The expression of CD68 in eutopic endometrial in adenomyosis was positively correlated with the number of IL-6 （r=0.303,P=0.021）.Conclusion Macrophage and IL-6 may play an important role in the pathogenesis of AM.