骨桥蛋白在原发免疫性血小板减少症患者血浆中的表达及意义

目的通过检测原发免疫性血小板减少症（ITP）患者治疗前后及正常人外周血血浆骨桥蛋白（OPN）含量，初步探讨OPN在ITP发病中的意义。 方法收集40例健康成年人外周静脉血5 ml作为对照组，收集40例ITP患者治疗前及治疗后外周静脉血各5 ml作为治疗前及治疗后组。标本离心后收集血浆，用骨桥蛋白酶联免疫吸附法酶联免疫试剂盒测量标本中OPN含量，观察对照组、治疗前组、治疗后组OPN的表达情况，并分析初治ITP患者血小板计数与血浆OPN表达水平的相关性及治疗有效组与无效组在治疗前后OPN含量变化的差别。 结果ITP治疗前组血浆OPN水平高于对照组血浆OPN水平，差异有统计学意义（P<0.05），ITP治疗后组血浆OPN水平与治疗前组比较呈下降趋势，差异具有统计学意义（P<0.05）。ITP初治患者血小板计数和血浆OPN表达呈负相关（r=-0.80），差异具有统计学意义（P<0.05）。40例ITP患者治疗后有效率为92.5%（37/40），无效率为7.5%（3/40），有效组治疗前后OPN的变化高于无效组，差异具有统计学意义（P<0.05）。 结论OPN可能参与了ITP的致病过程，其与血小板减少的程度具有一定相关性，监测治疗前后OPN含量的变化或许可为评估ITP疗效提供参考。

Significance of expression of osteopontin in plasma of patients with primary immune thrombocytopenia

ObjectiveTo explore the role of OPN in the pathogenesis of immune thrombocytopenia (ITP) by detecting the content of plasma osteoponetontin (OPN) in patients with primary ITP before and after treatment and in normal controls. MethodsPeripheral venous blood (5 ml) was collected from 40 healthy adults (control group) and 40 ITP patients before and after treatment. The collected samples were centrifuged to separate the plasma. Enzyme-linked immunosorbent assay (ELISA) was used to measure the content of OPN in the samples to observe the expression of OPN in the control group, the pre-treatment group, and the post-treatment group. The correlation was analyzed between platelet count and plasma OPN expression in ITP patients, and OPN contents were compared between the effective group and the ineffective group before and after treatment. ResultsPlasma OPN level in the pre-treatment group was significantly higher than that in the control group (P<0.05). Plasma OPN level in the post-treatment group showed a significantly decreasing trend compared with that the pre-treatment group (P<0.05). There was a significant negative correlation between platelet count and plasma OPN expression in ITP patients (r=-0.80, P<0.05). After treatment, the effective rate was 92.5% (37/40) and the ineffective rate was 7.5% (3/40). The OPN change between before and after treatment was significantly higher in the effective group than in the ineffective group (P<0.05). ConclusionOPN may be involved in the pathogenesis of ITP, which is correlated with the degree of thrombocytopenia. Monitoring the change of OPN content between before and after treatment may provide a reference for evaluating the therapeutic efficacy for ITP.