MicroRNAs 的失调在高转移肝细胞癌中的作用

目的：筛选在高转移肝细胞癌中差异表达的miRNAs图谱。方法利用Agilent miRNA array芯片技术平台分析比较了4对高转移肝细胞患者的癌组织样本与其相对应的正常组织之间的差异miRNAs。通过生物信息学方法分析了这些候选差异 miRNAs。结果与正常组织相比，我们在高转移肝细胞癌中发现了22个失调的miRNAs，其中包括13个上调的miRNAs（miR-200a、miR-425、miR-221和miR-20b等）和9个下调的miRNAs（miR-762、miR-638和miR-1305等）。其中，一些miRNAs已经被报道与肿瘤的发生和转移相关。靶基因预测分析也表明，这些基因在肿瘤的发生和转移过程中扮演着重要的作用。结论我们在高转移肝细胞癌中发现了一些表达失调的 miRNAs，生物信息学分析也发现这些 miRNAs 在肿瘤的发生和转移中发挥着重要的作用，可以作为肝细胞癌在临床治疗上的一个新的肿瘤标志物。

The role of deregulated microRNAs in high metastatic hepatocellular carcinoma

Objective To identify the differentially expressed in the high metastatic hepatocellular carcinoma（HCC）. Methods By the Agilent human miRNA array, we compare the miRNA expression profile between four pairs high metastatic HCC tissues and their normal counterpart. Bioinformatics analysis was also performed. Results A total of 22 dysregulated miRNAs were identified in high metastatic HCC compared to their normal counterpart, including 13 upregulated miRNAs（miR-200a, miR-425, miR-221 and miR-20b, etc） and 9 downregulated miRNAs（miR-762, miR-638 and miR-1305, etc）. Some of these miRNAs have been reported to be dysregulated in other malignancies and have a potential role in cancer metastasis. Target genes prediction also showed these targets can play important roles in HCC pathogenesis and metastasis. Conclusions We identified miRNAs that are dysregulated in high metastatic HCC and bioinformatics analysis suggests that these miRNAs may be involved in cancer pathogenesis and metastasis and have the potential to be biomarkers.