曲古抑菌素A增强细胞因子诱导的杀伤细胞对胰腺癌细胞株杀伤效果的体外研究

目的：研究细胞因子诱导的杀伤（CIK）细胞对胰腺癌细胞株的杀伤效果；探索曲古抑菌素A（TSA）增强其杀伤效果的机制。方法将胰腺癌细胞株分为2组。一组经100 mmol/L；200 mmol/L浓度的TSA处理24 h，另外一组为空白对照。分别使用流式细胞仪测定两组胰腺癌细胞株的MICA/B表达及使用乳酸脱氢酶法测定CIK细胞对两组胰腺癌细胞株的杀伤率。双变量相关性分析胰腺癌细胞株MICA/B的表达与CIK细胞对胰腺癌细胞株杀伤率的关系。结果经TSA处理24 h后的胰腺癌细胞株，其MICA/B表达相较对照组有明显的升高（P＝0.000），有统计学差异；在不同靶效比的情况下，CIK 细胞对经 TSA处理24 h后的胰腺癌细胞株的杀伤率有显著的提高（P＝0.000）；而相关性分析显示，CIK细胞对胰腺癌细胞株杀伤率的升高与胰腺癌细胞株MICA/B的表达呈正相关（P＜0.01）。结论 TSA可显著调高胰腺癌细胞株的MICA/B表达（P＜0.01），从而增强CIK细胞对胰腺癌细胞株的杀伤。

The in-vitro studies of TSA enhanced killing effects of CIK to pancreatic cancer cell lines

Objective To study on pancreatic cancer cell lines CIK killing effect;explore TSA mechanisms to enhance its lethal effect. Methods Pancreatic cancer cell lines are divided into two groups. One group was the cell of 100 mmol/L; 200 mmol/L for 24 hours TSA concentration, the other group was the control group. Respectively, flow cytometry determined two group pancreatic cancer cell lines MICA/B expression; of lactate dehydrogenase methods measured the killing rates of two groups of CIK to pancreatic cancer cell lines. Bivariate correlation analysis of the relationship of the MICA/B expression in pancreatic cancer cell lines and the CIK killing rates. Results Using TSA for 24 hours, the MICA/B expression of pancreatic cancer cell lines increased significantly, which compared to the control group （P=0.000）, with statistically significant differences. CIK killing rates of pancreatic cancer cell lines was significantly increased which was using TSA treatment for 24 hours （P=0.000）;Bivariate correlation showed that, the relationship of CIK killing rates of pancreatic cancer cell lines and the MICA/B expression was positive correlation （P＆lt;0.01）. Conclusion TSA can significantly increase the MICA/B expression of pancreatic cancer cell lines （P＆lt;0.01）, thereby enhancing CIK killing rates of pancreatic cancer cell lines.