冬凌草甲素对人膀胱癌T24和5637细胞杀伤作用的研究

目的]探讨冬凌草甲素对不同恶性程度人膀胱癌细胞的抑制作用.方法]以丝裂霉素为对照,用不同浓度的冬凌草甲素处理人膀胱癌细胞株T24(浸润性,G3)和5637(非浸润性,G2)后,通过MTT法检测细胞增殖抑制率并计算IC50值,应用倒置显微镜观察细胞形态及其抑制情况,采用荧光染色法观察细胞核及细胞凋亡情况.结果]冬凌草甲素和丝裂霉素对T24和5637细胞均有抑制增殖和杀伤作用,并呈现时间-效应和浓度-效应关系.分别作用T24、5637细胞株12 h和24 h 后,64 μmol/L冬凌草甲素的抑制增殖和杀伤效果均要显著优于150 μmol/L丝裂霉素的效果.低恶性细胞5637较高恶性T24对冬凌草甲素和丝裂霉素处理更敏感.结论]冬凌草甲素能通过诱导T24和5637细胞凋亡,起到杀伤和抑制增殖的效果,且较丝裂霉素起效快,有进一步研究的价值.

Cytotoxicity of Oridonin on Human Bladder Cancer T24 and 5637 Cells

Objective]To investigate the the cytotoxicity of oridonin on different malignant types of human bladder cancer cells.Methods]With mitomycin as a control, different concentrations of oridonin were treated to human bladder cancer T24 (invasive, G3) and 5637 (non-invasive, G2) cells.Cell proliferation inhibition was measured by MTT assay and IC50 values were calculated for comparison.Cell morphologic changes and apoptosis were observed by inverted microscope and fluorescence staining.Results]Oridonin and mitomycin inhibited cell proliferation and killed both T24 and 5637 cells, both showing time-and concentration-dependent effects.The proliferation inhibition and killing effects of oridonin at 64μM were significantly better than those of mitomycin at 150μM after treating T24 and 5637 cells for 12 h and 24 h.Compared with highly malignant invasive bladder cancer cells T24, low malignancy non-invasive bladder cancer cells 5637 were more sensitive to oridonin and mitomycin treatment.Conclusion] Compared with mitomycin, oridonin induces apoptosis, inhibits proliferation, and kills bladder cancer T24 and 5637 cells to a greater extend.It is worthy of further research and development.