凋亡因子Apaf-1在大肠肿瘤中的表达及其与Caspase-3及Ki-67相关性研究

目的探讨 Apaf-1在大肠癌中的表达及其与Caspase-3及Ki-67的相关性。方法收集大肠癌（肠癌组）、腺瘤（腺瘤组）及正常黏膜组织（正常组）标本,每组各100例,免疫组化 SP 染色方法检测 Apaf-1表达,并分析其与Caspase-3、Ki-67相关性。结果肠癌组、腺瘤组及正常组中,Apaf-1蛋白的阳性表达率分别为39.00%、88.00%、95.00%；Caspase-3蛋白的阳性表达率分别为45.00%、83.00%、91.00%,肠癌组中 Apaf-1、Caspase-3阳性率低于腺瘤组及正常组（P ＜0.05）；Ki-67蛋白的阳性表达率分别为68.00%、26.00%、8.00%,肠癌组中Ki-67阳性率高于腺瘤组及正常组（P＜0.05）。Apaf-1与Caspase-3阳性表达呈正相关（r=0.345,P ＜0.01）,与Ki-67阳性表达呈负相关（r=-0.361,P ＜0.01）。结论 Apaf-1、Caspase-3、Ki-67均参与了大肠癌的发展过程,推测大肠癌患者 Apaf-1受到抑制,进而Caspase-3活化减少,肿瘤细胞增加。

Expression of Apoptosis Factor Apaf-1 in Large Intestinal Cancer and Its Relationship with Caspase-3 and Ki-6 7

Obj ective]To explore the expression of apoptosis factor Apaf-1 in large intestinal cancer and its relationship with Caspase-3 and Ki-67.Methods]The specimens of 100 cases of large intestinal cancer（intestinal cancer group）,100 cases of adenoma（adenoma group）and 100 cases of normal mucosa（normal group）were col-lected.Immunohistochemical SP method was used to detect the expression of Apaf-1 .The relationship of the ex-pression of Apaf-1 with Caspase-3 and Ki-67 was analyzed.Results]The positive expression rates of Apa-1 pro-tein in intestinal cancer group,adenoma group and normal group were 39.00%,88.00% and 95.00% respective-ly,while the positive expression rates of Caspase-3 protein were 45.00%,83.00% and 91.00%,respectively. The positive expression rates of Apaf-1 and Caspase-3 in intestinal tumor group were lower than those in adenoma group and normal group（P〈0.05）.The positive expression rates of Ki-67 protein in intestinal cancer group,ad-enoma group and normal group were 68.00%,26.00% and 8.00% respectively,and that in intestinal cancer group was higher than those in adenoma group and normal group（P 〈0.05）.The positive expression of Apaf-1 was positively correlated with the positive expression of Caspase-3（r=0.345,P〈0.01）,and negatively correla-ted with the positive expression of Ki-67（r=-0.361,P〈0.01）.Conclusion]Apaf-1,Caspase-3 and Ki-67 in-volve in the development of large intestinal cancer which infers that Apaf-1 in patients with large intestinal cancer is inhibited,thus the activation of Caspase-3 is reduced and tumor cell is increased.