食管癌组织miR-133表达对其癌细胞生长的影响

【目的】探讨食管癌组织中miR-133表达对其癌细胞生长的影响。【方法】采用RT-PCR法检测miR-133在食管癌组织中的表达。利用脂质体Lipofectamine 2000将miR-133 mimics、anti-miR-133及其分别相应的对照转入食管癌细胞中,RT-PCR检测miR-133的表达,MTT法检测细胞增殖能力,Western blot检测细胞中EGFR蛋白的表达。【结果】miR-133在食管癌组织中的相对表达量(0.33±0.08)显著低于其癌旁正常组织(1.15±0.18)。miR-133过表达显著抑制了食管癌细胞的生长,而miR-133的下调显著增强了肿瘤细胞的生长。同时,miR-133的过表达抑制了EGFR mRNA和蛋白的表达,miR-133的下调显著增强了EGFR mRNA和蛋白的表达。EGFR的过表达逆转了miR-133对食管癌细胞生长的抑制效应。【结论】miR-133通过调控EGFR的表达进而抑制食管癌细胞的生长。

Effect of miR-133 Expression on the Growth of Esophageal Cancer Cells

【Objective】To investigate the effect of miR-133 expression on the growth of esophageal cancer cells.【Methods】The expression of miR-133 in esophageal carcinoma was detected by RT-PCR,miR-133 mimics,anti-mir-133 and their corresponding controls were transfected into esophageal cancer cells by Lipofectamine 2000.RT-PCR was used to detect the expression of miR-133,MTT was used to detect the cell proliferation,and Western blot was used to detect the expression of EGFR protein.【Results】The relative expression of miR-133 in esophageal carcinoma tissues(0.33±0.08)was significantly lower than that in adjacent normal tissues(1.15±0.18).Overexpression of miR-133 significantly inhibited the growth of esophageal cancer cells,while down-regulation of miR-133 significantly enhanced the growth of tumor cells.Meanwhile,overexpression of miR-133 inhibited the expression of EGFR mRNA and protein,and down-regulation of miR-133 significantly enhanced the expression of EGFR mRNA and protein.Overexpression of EGFR reverses the inhibitory effect of miR-133 on the growth of esophageal cancer cells.【Conclusion】miR-133 inhibits the growth of esopha-geal cancer cells by regulating the expression of EGFR.