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核酸扩增技术在上海血液筛检中的初步应用
引用本文:王迅,郑岚,张晰,凌冰,许莉萍,黄宇闻,陆萍,莫琴,韩永年,高峰.核酸扩增技术在上海血液筛检中的初步应用[J].中国输血杂志,2003,16(3):157-160.
作者姓名:王迅  郑岚  张晰  凌冰  许莉萍  黄宇闻  陆萍  莫琴  韩永年  高峰
作者单位:1. 上海市血液中心,上海,200051
2. 上海市第八人民医院
摘    要:目的 为正确评估目前酶免抗体筛检方法的血液安全性,探讨在我国血液筛检中引进核酸扩增技术(NAT),防止酶免抗体筛检方法窗口期漏检的可行性。方法 使用Procleix~(TM)TMA HIV-1/HCV检测方法,分2个阶段,分别以8份样品和24份样品混合的形式,总共筛选了103539份上海市区的献血标本。结果 发现5份第2次酶免检测(EIA-2)HCV抗体和NAT同时阳性的标本,同时也发现275份(0.27%)EIA-2 HCV抗体阳性但NAT阴性标本和107份(0.10%)EIA-2 HIV抗体阳性但NAT阴性的标本,未发现血清抗体转换窗口期标本。结论 上海市的血液安全水平目前已接近发达国家水平,HCV和HIV的感染危险性已经小于1:100000,Procleix~(TM)TMAHIV-1/HCV检测技术具有较高的敏感性和特异性,适合在中国血液筛检中应用。

关 键 词:核酸扩增技术  血液筛选  上海
文章编号:1004-549X(2003)03-0157-04
修稿时间:2002年7月8日

Preliminary experiences of nucleic acid testing in blood screening in Shanghai,China
Wang Xun,Zheng Lan,Zhang Xi,et al..Preliminary experiences of nucleic acid testing in blood screening in Shanghai,China[J].Chinese Journal of Blood Transfusion,2003,16(3):157-160.
Authors:Wang Xun  Zheng Lan  Zhang Xi  
Institution:Wang Xun,Zheng Lan,Zhang Xi,et al. Shanghai Blood Center,Shanghai 200051,China
Abstract:Objective To assess the blood safety under the current sero-screening program and gain experience on how to implement NAT as a routine assay for blood screening in China. Methods Sera from 103,539 non-remunerated donors of Shanghai blood center were tested by Chiron's Procleix(TMA HIV-1/HCV Assay in 2 study stages with 8 and 24 sample-pool respectively. Results 5 NAT positive samples were found to be Anti-HCV EIA-2 test positive at the same time. No window period cases of HIV-1/HCV were found in this donor group. But 275 (0.27% ) anti-HCV and 107 (0. 10%) anti-HIV false positive samples were identified by the second sero-screening. Conclusion The blood quality of Shanghai city was high to be close to the level of developed countries and the window period risk of HIV-1 and HCV was less than 1:100,000. With its high sensitivity and specificity,Procleix( TMA HIV-1/HCV Assay can be used in routine blood screening in China.
Keywords:Nucleic acid testing  Blood screening
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