一氧化氮供体改善血小板保存质量的初步研究

目的探讨一氧化氮(NO)供体S-亚硝基乙酰青霉胺(SNAP)对常温保存血小板过程中血小板质量的影响。方法离心法制备浓缩血小板共12人份,38~40 ml/份。将相同血型的2袋混合,加入复温后的冰冻血浆至约100 ml,混匀后均分、转移至2个血小板专用保存袋,分别为实验组:保存前加入终浓度10~5mol/L SNAP;对照组:加入等体积的无菌生理盐水。(22±2)℃振荡保存7 d,分别在d1、d3、d5、d7取样检测血小板计数、pH、血小板活化率及抗低渗性休克反应等指标。结果 2组血小板在保存过程中pH均保持在6.8以上;血小板活化率均不断升高,实验组从(5.93±1.43)%升高到(44.22±6.84)%,对照组从(8.22±1.33)%升高到(54.32±5.68)%,d1、d3、d5、d7 2组血小板之间活化率差异有统计学意义(P<0.05);d1、d5、d7实验组血小板抗低渗休克反应分别为(65.98±7.57%)、(53.1±8.44)%、(44.23±0.08)%,对照组为(50.92±4.48)%、(40.06±4.66)%、(35.28±0.04)%,d1、d5、d7实验组抗低渗休克反应能力高于对照组,差异具有统计学意义(P<0.05)。结论在血小板保存过程中加入NO供体SNAP一定程度上可以抑制血小板活化,改善血小板功能。

Improvement on the quality of storage platelets with Nitric Oxide donor

Objective To evaluate the impact of nitric oxide donor S-nitroso-N-acetylpenicillamine(SNAP) on the quality of platelet stored at(22±2)℃.Methods Whole blood from 12 persons were centrifuged to prepare 12 bags of platelet concentrates(PC) according to the standard operating procedures,with 38~40 ml of PC in each bag.Two bags of PCs in the same ABO blood group were mixed.Dissolved fresh frozen plasma was then added to the mixed PCs to achieve the final volume of 100ml.Each mixture was divided into two equal parts and were transferred to platelet-specific storage bags,one taken as the experimental group,the other as the control.SNAP(final concentration:10-5mol/L) was added to experimental group before storage,and an equivalent amount of sterile saline was added to the control group.All PCs were stored at(22±2)℃ for 7 days.Platelet count,pH,CD62p,and hypotonic shock response were detected on the 1 st,3 rd,5 th and the 7 th day of storage.Results During 7 days of storage,the pH of both groups were higher than 6.8.The rates of platelet activation were on the rise:the experimental group from(5.93 ± 1.43)% to(44.22 ± 6.84)% while the control group from(8.22 ± 1.33)% to(54.32 ± 5.68)%,with significant difference between the two groups(P<0.05) on the 1st,3rd,5th and 7th day.Anti-HRS rates of the experimental group on the 1 st,5 th,7 th day were(65.98±7.57)%,(53.1±8.44)%,(44.23±0.08)%,respectively,while those in the control group were(50.92±4.48)%,(40.06±4.66)%,and(35.28±0.04)%,respectively,with significant differences between the two groups(P<0.05).Conclusion The addition of SNAP into PCs can potentially alleviate storage lesions as well as improve platelet functions.