脉冲场凝胶电泳用于军团菌分型能力的评价

目的 评价脉冲场凝胶电泳(PFGE)技术用于军团菌属不同种(Legionella species)以及不同血清型菌株的分型能力.方法 选用117株10个嗜肺军闭菌血清型,30种非嗜肺军团菌的参考菌株和我国环境分离菌株,采用Asc Ⅰ内切酶酶切,进行PFGE.从电泳条带的数量和分布、PFGE的分型力、分辨率和与流行病学资...

Evaluation of pulsed-field gel electrophoresis for Legionella subtyping

Objective To evaluate the capability of pulsed-field gel electrophoresis（PFGE） for the subtyping of Legionella.Methods A total of 117 Legionella reference strains and environmental strains were analyzed by PFGE with Asc Ⅰ.Combined with the number and distributions of bands in the PFGE patterns,capability of subtyping,distinguishability of patterns and epidemiological information,we evaluated the capability of Asc Ⅰ-PFGE for Legionella subtyping.Results All Legionella pneumophila serotypes strains and 17 non-L.pneumophila strains showed high qualitative PFGE patterns;however,for other 13 non-L.pneumophila serotypes strains,most of the PFGE bands were distributed with small weight.Asc Ⅰ-PFGE could cluster the isolates from each water system into the same or very similar patterns and distinguish the strains with different sources.By Asc Ⅰ-PFGE,L.pneumophila subtyping had a high degree of consistency with epidemiological data of strains.Conclusion Asc Ⅰ-PFGE could be used in the subtyping of L.pneumophila and 17 non-L.pneumophila strains,and be applied in the epidemiological investigation of L.pneumophila disease.