C群脑膜炎奈瑟菌部分蛋白表型特征分析

目的以蛋白质组学研究为基础,分析2003～2005年间我国流脑暴发流行期间C群脑膜炎奈瑟菌菌株分布特征。方法利用双向电泳技术获得66株2003～2005年流脑流行期内分离的C群脑膜炎奈瑟菌菌株及2株参考菌株的2-DE电泳图谱,并结合质谱分析的结果进行部分外膜蛋白和sucD蛋白表型分析。结果根据外膜蛋白和sucD蛋白将实验菌株共分为9型,其中安徽菌株分型结果显示出了高度的一致性,而其他地区菌株分型分布则显示出高度的多态性。结论安徽已经有优势菌型形成,但优势菌型还没在全国范围内出现,此类菌型分布和变迁的检测可能对认识流脑的流行规律和有效进行流脑疾病控制具有重要意义。

Analysis of the phenotypic traits of some proteins of serogroup C Neisseria meningitidis strains

Objective This study was conducted to analyze the distribution characteristics of the serogroup C Neisseria meningitidis strains isolated during the epidemic outbreaks between 2003 and 2005 in China. Methods The electrophoresis patterns of sixty-six serogroup C Neisseria meningitidis strains isolated and 2 standard strains were obtained by the two-dimensional electrophoresis, and the analysis was made on the phenotypes of outer membrane proteins and sucD protein in combination of the results from the mass spectrometry. Results 9 types were developed depending on the variation of outer membrane proteins and sucD spot pattern in 2-DE maps. The spot patterns showed high similarities in strains isolated from Anhui Province, but were more diverse in strains isolated from other places. Conclusion One predominant strain type has developed in Anhui Province, but has not disseminated to all the other places. Detecting the distribution and transition of the strain types may be of importance in the cognition of the epidemic characteristics of Neisseria meningitides and in the control and prevention of epidemic cerebrospinal meningitis in the future.