深圳东门市场野生动物SARS病毒的监测

目的　对深圳东门市场野生动物标本中SARS病毒进行监测 ,了解动物携带SARS病毒的情况。方法　对 2 0 0 3年 5月 7日至 12月在东门市场采集的果子狸、貉、鼬獾、猪獾和狗獾等 5种动物标本 ,进行病毒分离鉴定及RT -PCR和SARS荧光定量PCR检测。结果　 5月首先从 4份果子狸和 1份貉标本中分离到SARS病毒。 5月至 12月的 12 1份动物标本中共有 5 5份SARS病毒阳性标本 ,阳性率为 45.45 %。 5月份、10月份、11月份和 12月份野生动物标本中SARS阳性率分别为 50%、83.33%、34.78%和 44.87% ,其中 ,果子狸的SARS病毒携带率为 90 %。结论　东门市场售卖的果子狸可能是携带SARS病毒的重要载体之一 ,在SARS的传播中可能起着重要的作用。

Surveillance of SARS Coronavirus among wild animal sold in Dongmen Market in Shenzhen City

Objective To survey SARS Coronavirus among wild animals sold in Dongmen Market and to learn the carried rate of SARS Coronavirus.Methods Samples of civet cat,raccoon-dog,melogalemoschata,arctonyx collaris and meles were collected from Dongmen Market from 7th May to December in 2003.SARS-Cov were isolated,and were detect by fluorescence PCR and RT-PCR.Results SARS-Cov were isolated from four civet cats and one raccoon-dog in May.In 121 animal samples,55 were SARS-Cov positive from May to December,the positive rate was 45.45%. In May,October,November and December,the positve rate of SARS-Cov for wild animal samples were 50%,83.33%,34.78% and 44.87%,respectively. The SARS-Cov carried rate of civet cats was 90%.Conclusion Civet cat sold in Dongmen market was probably one of significant carriers for SARS-Cov,and was playing an important role in SARS epidemic.