| 弗氏枸橼酸杆菌CF74的T6SS影响FliC的表达和分泌 |
| |
| 引用本文： | 王艺婷,郝帅,肖迪,叶长芸,熊衍文,刘丽云.弗氏枸橼酸杆菌CF74的T6SS影响FliC的表达和分泌[J].疾病监测,2016,31(3):185-191. |
| |
| 作者姓名： | 王艺婷 郝帅 肖迪 叶长芸 熊衍文 刘丽云 |
| |
| 作者单位： | 1.中国疾病预防控制中心传染病预防控制所, 传染病预防控制国家重点实验室, 北京 102206 |
| |
| 基金项目： | This study were supported by the funds from National Natural Science Foundation of China (No.81301401) and for the Surface Project of State Key Laboratory for Communicable Disease Prevention and Control (No.2011SKLID209) ，国家自然科学基金(No.81301401)，传染病预防控制国家重点实验室面上项目(No.2011SKLID209) |
| |
| 摘 要： | 目的 研究弗氏枸橼酸杆菌CF74的T6SS基因组岛功能。方法 构建弗氏枸橼酸杆菌CF74的T6SS基因组岛的精确缺失突变株(CF74T6SS)。对弗氏枸橼酸杆菌CF74和其突变株CF74T6SS的全菌蛋白和上清蛋白进行提取,并进行2-DE分析。结果 在CF74T6SS全菌蛋白中,有4个蛋白表达上调,42个蛋白表达下调,并且FliC的表达下调十分明显;在CF74T6SS的培养上清中,有27个蛋白表达上调,36个蛋白表达下调,并且FliC、FlgK和FliD的表达下调十分明显。结论 弗氏枸橼酸杆菌CF74的T6SS可以影响FliC蛋白的表达和分泌。
|
| 关 键 词： | 弗氏枸橼酸杆菌 CF74T6SS 2-DE FliC |
| 收稿时间： | 2015-11-20 |
T6SS modulates expression and secretion of FliC in Citrobacter freundii |
| |
| Institution: | 1.State Key Laboratory for Communicable Disease Prevention and Control, Institute for Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China2.School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China |
| |
| Abstract: | Objective To screen fuction of CF74 T6SS genomic island. Methods We constructed deletion mutant of T6SS genomic island, and used 2-DE to compare the proteins in the WCL and culture supernatants from bacteria grown to stationary phase in LB between the wild-type and CF74T6SS mutant. Results In WCL, 4 and 42 protein-coding genes exhibited higher or lower levels of expression in CF74T6SS, respectively. FliC was found to have markedly decreased production in CF74T6SS. In culture supernatants, we identified 27 up-regulated and 36 down-regulated proteins in CF74T6SS. Three flagellar proteins, FliC, FlgK and FliD were identified, which were expressed less in CF74T6SS. Conclusion The absence of T6SS reduced the production and secretion of FliC and at least 2 other flagellar proteins. |
| |
| Keywords: | Citrobacter freundii CF74△T6SS 2-DE FliC |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《疾病监测》浏览原始摘要信息 |
| 点击此处可从《疾病监测》下载免费的PDF全文 |
|
