| 大鼠骨髓间充质干细胞的超顺磁氧化铁颗粒标记及MR成像 |
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| 引用本文： | 周斌,于春鹏,李丹,姜在波,钱结胜,朱康顺,庞鹏飞,单鸿.大鼠骨髓间充质干细胞的超顺磁氧化铁颗粒标记及MR成像[J].中国医学影像技术,2008,24(12):1879-1882. |
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| 作者姓名： | 周斌 于春鹏 李丹 姜在波 钱结胜 朱康顺 庞鹏飞 单鸿 |
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| 作者单位： | 1. 中山大学附属第三医院放射科,广东,广州,510630
2. 青岛大学医学院附属医院介入放射科,山东,青岛,266011 |
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| 基金项目： | 国家自然科学基金
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广东省科技厅科学事业费计划
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| 摘 要： | 目的描述超顺磁氧化铁(SPIO)颗粒标记大鼠骨髓间充质干细胞(BMSCs)后对细胞的影响及MR成像特点,评价其标记效果。方法分离、纯化并培养大鼠BMSCs,使用多聚赖氨酸(PLL)对细胞进行SPIO(50μgFe/ml,48h)标记,采用普鲁士蓝染色、细胞内铁含量检测等评定SPIO标记BMSCs的效率;通过台盼蓝染色、二甲基亚砜比色法检测细胞活力和增殖情况,通过Hoechst染色观察细胞凋亡情况,评价SPIO是否影响BMSCs的生物学特性;并对标记的BMSCs进行MR成像(序列:T1SE、T2FSE及T2*GRE)。结果细胞标记率达100%,标记细胞铁含量明显高于未标记细胞;细胞活力、增殖、周期和凋亡检测显示标记细胞与未标记细胞间无统计学差异(P>0.05);MR成像显示标记细胞数目大于5×103个细胞时,3个序列图像均能够观察到信号变化,以T2*WI最为明显。结论大鼠BMSCs可以被SPIO(50μgFe/ml,48h)有效标记,且对细胞的生物学特性无明显影响;1.5TMR能够在体外观察到标记后的细胞。
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| 关 键 词： | 间充质干细胞 超顺磁氧化铁 磁共振成像 |
| 收稿时间： | 9/5/2008 12:00:00 AM |
| 修稿时间： | 2008/9/24 0:00:00 |
Superparamagnetic iron oxide labeling and MR imaging of rat bone marrow mesenchymal stem cells in vitro |
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| ZHOU Bin,YU Chun-peng,LI Dan,JIANG Zai-bo,QIAN Jie-sheng,ZHU Kang-shun,PANG Peng-fei and SHAN Hong.Superparamagnetic iron oxide labeling and MR imaging of rat bone marrow mesenchymal stem cells in vitro[J].Chinese Journal of Medical Imaging Technology,2008,24(12):1879-1882. |
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| Authors: | ZHOU Bin YU Chun-peng LI Dan JIANG Zai-bo QIAN Jie-sheng ZHU Kang-shun PANG Peng-fei and SHAN Hong |
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| Institution: | Department of Radiology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China;Department of Interventional Radiology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266011, China;Department of Radiology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China;Department of Radiology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China;Department of Radiology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China;Department of Radiology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China;Department of Radiology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China;Department of Radiology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China |
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| Abstract: | |
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| Keywords: | Mesenchymal stem cells Superparamagnetic iron oxide Magnetic resonance imaging |
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