局灶亚低温对创伤性脑损伤后大鼠脑组织炎症反应的影响

目的 观察创伤性脑损伤后早期应用局灶亚低温治疗对脑组织炎症反应的影响,探讨其治疗创伤性脑损伤的机制.方法 采用自由落体撞击模型,63只雄性Sprague Dawley大鼠随机分为对照组、颅脑外伤组及局灶亚低温组(使用25℃水降温),每组再根据伤后不同生存时间随机分为3个亚组(每组7只).取伤灶脑组织检测髓过氧化物酶(MPO)活性,做细胞间黏附分子-1(ICAM-1)免疫组化染色,光镜下计数ICAM-1阳性血管数.数据的统计采用SPSS 10.0软件行SNK-q检验.结果 局灶亚低温组各时间点伤灶区ICAM-1阳性血管数均明显低于颅脑外伤组相应时间点(P<0.01).局灶亚低温组各时间点MPO活性均明显低于颅脑外伤组相应时问点(P<0.01).结论 局灶亚低温可减少伤灶区ICAM-1的表达及中性粒细胞浸润,这可能是其治疗创伤性脑损伤的机制之一.

Effects of focal mild hypothermia on inflammation after traumatic brain injury in rats

Objective To study the effects and mechanism of focal mild hypothennia on brain inflarnmation response during the early stage of tranrntic brain injury(TBI)in rats.Method Traumatic brain injury wag produced in anesthetized rats by using free-falling impact model.Sixty-three male Sprague Dawley rats were randomly divided into three groups:TBI group,focal hypothermia group,and sham group.each group with 7 SD rats.Then all groups Wag further subdivided into three subgroups according to different intervals after TBI.Myeloperoxiduse (MPO)activity assay,immunohistochemical staining for the detennination of intercellular adhesion molecule-1(ICAM-1)expression and the number of ICAM-1 positive blood vessels were counted up in the traumatic brain tissues.All data were analyzed by SNK-q test Using SPSS 10.0 sofeware program.Results The number of ICAM1 positive blood vessels was significantly fewer in the rats of focal hypothermia group than those in the rats of TBI group at different intervals after TBI(P<0.01).Similarly,the MPO activity was significantly lower in the rats of focal hypothermia group than that in the rats of TBI group(P<0.01).Conclusions Focal mild hypothermia could significantly decrease neutrophil infdtration and ICAM-1 expression in the traumatized areas.It might be one of the mechanisms of focal mild hypothermis for TBI.