| 严重颅脑损伤后细胞凋亡状态及相关基因Bcl-2、Bax的表达 |
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| 引用本文： | 杨小锋,李谷,刘伟国,林敏.严重颅脑损伤后细胞凋亡状态及相关基因Bcl-2、Bax的表达[J].中华急诊医学杂志,2004,13(2):100-102. |
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| 作者姓名： | 杨小锋 李谷 刘伟国 林敏 |
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| 作者单位： | 310009,杭州,浙江大学医学院附属第二医院神经外科 |
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| 基金项目： | 浙江省医学科研基金 ( 2 0 0 0A110 ) |
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| 摘 要： | 目的 探讨严重颅脑损伤后Bel-2、Bax基因表达与细胞凋亡的关系。方法 采用改进的Feeney自由落体损伤模型，应用分子生物学方法——脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(terminal-deoxynucleofidyl transferase mediated nick end labeling，TUNEL)以及免疫组织化学方法，对50只严重颅脑大鼠脑组织中的凋亡细胞和Bel-2、Bax免疫反应阳性的细胞进行观察、统计。结果 损伤组大鼠脑组织中凋亡细胞数明显高于未损伤组，Bel-2、Bax表达也有所增加，同时，损伤组Bax／Bel-2阳性细胞数的比值明显大于未损伤组。结论 Bel-2和Bax表达的比例对严重颅脑损伤后细胞凋亡可能起重要的调控作用。
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| 关 键 词： | 严重颅脑损伤 细胞凋亡 Bcl-2 BaX 基因表达 |
| 修稿时间： | 2003年4月17日 |
The expression of Bcl-2,Bax and apoptosis after severe brain injury |
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| YANG Xiaofeng,LI Gu,LIU Weiguo,et al..The expression of Bcl-2,Bax and apoptosis after severe brain injury[J].Chinese Journal of Emergency Medicine,2004,13(2):100-102. |
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| Authors: | YANG Xiaofeng LI Gu LIU Weiguo |
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| Institution: | YANG Xiaofeng,LI Gu,LIU Weiguo,et al.The Second Hospital Affiliated to Zhejiang University,Hangzhou 310009,China |
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| Abstract: | Objective To discuss the relationship between expression of Bcl-2,Bax and apoptosis after severe brain injury. Methods 50 rats were experimented, which were injured in the brain. TUNEL (terminal-deoxynucleotidyl transferase mediated nick end labeling) and immunohistochemisty were used to detect apoptosis and Bcl-2, Bax expression in all the brain tissue.Results There were significant differences between the injury group and noninjury group with more TUNEL?Bcl-2 Bax positive cells in the injured rats. And in injured rats the value of Bax/Bcl-2 was larger than that in uninjured rats.Conclusion Disequilibrium between the Bcl-2 and Bax may be the reason of promoting apoptosis after severe brain injury. |
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| Keywords: | Brain injury Apoptosis Bcl-2 gene Bax gene |
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