人脐带间充质干细胞体外诱导分化为成纤维细胞

背景：脐带间充质干细胞具有多向分化能力，但其向成纤维细胞分化研究较少。目的：验证人脐带间充质干细胞向成纤维细胞的分化能力。方法：采用贴壁法分离脐带间充质干细胞，流式细胞仪分析其表面抗原。取第3代脐带间充质干细胞进行成脂成骨诱导分化，以碱性成纤维细胞生长因子诱导脐带间充质干细胞向成纤维细胞分化。结果与结论：贴壁法能稳定从脐带中分离出干细胞，脐带间充质干细胞极低表达 CD31、CD45、CD40、HLA-DR，强表达 CD29、CD90、CD44、CD105。脐带间充质干细胞成脂诱导后油红O染色显示胞浆中充满红色的油滴；成骨诱导后茜素红染色可在细胞密集区见红色的钙结节。碱性成纤维细胞生因子诱导后细胞表达Ⅰ型胶原明显高于对照组。提示贴壁法分离脐带间充质干细胞可靠、纯度高，碱性成纤维细胞生长因子可诱导脐带间充质干细胞向成纤维细胞分化。

Human umbilical cord mesenchymal stem cells are induced in vitro to differentiate into fibroblasts

BACKGROUND：The umbilical cord mesenchymal stem cells possess multipotent differentiation capacity, but less research focus on its differentiation into fibroblasts.OBJECTIVE：To investigate the capacity of human umbilical cord mesenchymal stem cells differentiating into fibroblasts. METHODS：Using adherent method, human umbilical cord mesenchymal stem cells were isolated, and flow cytometric analysis of the surface antigen was performed. Passage 3 cells were selected for osteogenic and adipogenic differentiation, and cells differentiated into fibroblasts under the induction of basic fibroblast growth factor. RESULTS AND CONCLUSION：Adherent stem cells were stably isolated from the umbilical cord. Human umbilical cord mesenchymal stem cells lowly expressed CD31, CD45, CD40, HLA-DR, but strongly expressed CD29, CD90, CD44, CD105. Oil red O staining showed red droplets were ful of the cytoplasm after adipogenic induction;alizarin red staining showed red calcium nodules after osteogenic induction. After induced by basic fibroblast growth factor, the type I col agen expression was significantly higher than that in the control group. These findings indicate that the adherent human umbilical cord mesenchymal stem cells are reliably isolated with high purity;basic fibroblast growth factor can induce differentiation of umbilical cord mesenchymal stem cells into fibroblasts.