植入式皮质电刺激缺血性脑卒中大鼠皮质神经丝蛋白200的表达

背景：前期研究中发现单独皮质电刺激治疗16d能改善脑卒中后的运动功能障碍,增加梗死灶周围皮质微管相关蛋白2的表达。目的：观察植入式皮质电刺激对缺血性脑卒中模型大鼠皮质神经丝蛋白200表达的影响。方法：建立SD大鼠右侧大脑中动脉缺血性脑卒中模型,随机分成电刺激组和非刺激组,两组造模1周后植入电刺激器,电极放置在脑梗死区边缘皮质表面,电刺激组施加电刺激,非刺激组不施加电刺激。植入14d后终止电刺激,6周后用免疫荧光染色和免疫荧光图像分析系统定量分析梗死灶周边、梗死对侧皮质神经丝蛋白200的表达水平。结果与结论：与非刺激组比较,电刺激组梗死灶周边、梗死对侧皮质神经丝蛋白200表达水平均较高（P〈0.05）。表明皮质电刺激可增加梗死灶周边及梗死对侧皮质神经丝蛋白200的表达,诱导双侧皮质神经元轴突的可塑性改变。

Effect of cortical electrical stimulation on neurofilament protein 200 expression in ischemic stroke rats

BACKGROUND：Early studies have shown that cortical electrical stimulation（CES） alone for 16 days can enhance dyskinesia and the expression of microtubule-associated protein 2（MAP2） in peri-infarct cortex after cerebral ischemia in rats.OBJECTIVE：To investigate the effects of CES on the expression of neurofilament protein 200（NF-200） in rats model of ischemic stroke.METHODS：Sprague-Dawley rats model of right middle cerebral artery occlusion in ischemic stroke were established.The rats with lesions in the cortex were randomly divided into CES group（n=13） and non-stimulation group（n=10）.One week after modeled,electrical stimulators were implanted and the electrodes were placed on the surface of peri-infarct cortex.CES group was given the electric stimulation,but not in the non-stimulation group.CES was terminated after implantation for 14 days.And then the expression of NF-200 in the peri-infarct and contralateral cortex was assessed by immunofluorescence image analysis at 6 weeks after CES started.RESULTS AND CONCLUSION：In the peri-infarct and contralateral cortex,the expression of NF-200 in the CES group was higher than that in the non-stimulation group（P0.05）.These results suggest that CES can increase the expression of NF-200 in both peri-infarct and contralateral cortex,and induce the plasticity of bilateral cortical neuron axons.