荧光定量 PCR 法检测乙肝病毒 DNA 和乙肝病毒标志物检测的临床价值分析

目的：探讨荧光定量 PCR 法（FQ-PCR）检测乙肝病毒 DNA（HBV-DNA）和乙肝病毒标志物（HBV-M）ELISA 法检测的临床价值。方法选取2013年4～12月在该院检测的653例乙肝患者，先采用 ELISA 法对其血液标本进行 HBV-M 模式定性检测，检测顺序为乙型肝炎病毒表面抗原（HBsAg）、乙肝表面抗体（HbsAb）、乙型肝炎 e 抗原（HbeAg）、乙型肝炎 E 抗体（Hbe-Ab）、乙肝表面核心抗体（HbcAb）；再采用 FQ-PCR 法进行 HBV-DNA 定量检测，观察不同 HBV-M 模式检测结果。结果 HB-sAg、HbeAg、HbcAb 检测同时阳性简称“大三阳”。大三阳1（＋）、3（＋）、5（＋）模式]和1（＋）、3（＋）模式]的 HBV-DNA 阳性率分别为97．97％、94．74％，显著高于其他模式的 HBV-DNA 阳性率（P ＜0．05）。大三阳的 HBV-DNA 表达水平（5．59×10^6±2．42×10^5）copies/mL，明显高于其他模式（P ＜0．05）。结论联合 HBV-M 定性及 HBV-DNA 定量检测，对临床早期诊断及用药具有重要指导价值。

The clinical value of detection of fluorescence quantitative PCR in detection of HBV DNA and HBV markers

Objective To investigate the clinical value of detection of fluorescence quantitative PCR in detection of HBV DNA and HBV markers.Methods 653 hepatitis B patients in our hospital from 2013 April to 2013 December were selected.First ELISA method using HBV-M model for qualitative detection of the blood samples,the detection order：Hepatitis b virus surface antigen （HBsAg）,Hepatitis B surface Antibody（HbsAb）,Hepatitis B e Antigen（HbeAg）,hepatitis Be antibody（HbeAb）,hepatitis B core antibody（HbcAb）;Then the FQ-PCR method for the quantitative detection of HBV-DNA,different HBV-m model test results were compared..Results Big 3 this world1（＋）、3 （＋）、5 （＋）model]and1 （＋）、3 （＋）model ]of the HBV-DNA positive rate was 97.97%,94.74% was significantly higher than that in other mode（P〈0.05）.Big 3 this world1（＋）、3（＋）、5（＋）model]HBV-DNA expression level is the highest（5.59 ×10^6 ±2.42×10^5 ）copies/mL was significantly higher than that in other mode（P〈0.05）.Conclusion Combined with qualitative and quantitative detection of HBV-DNA HBV-M,which is of great value to clinical early diagnosis and therapy.