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大鼠弥漫性轴索损伤后神经行为变化和环孢霉素A的治疗作用
引用本文:尹卫东,许百男,王晓刚. 大鼠弥漫性轴索损伤后神经行为变化和环孢霉素A的治疗作用[J]. 中国危重病急救医学, 2004, 16(4): 214-217
作者姓名:尹卫东  许百男  王晓刚
作者单位:1. 100037,北京,海军总医院神经外科
2. 100853,北京,解放军总医院神经外科
基金项目:国家自然科学基金资助项目 (3 9870 793 7)
摘    要:目的 探讨大鼠弥漫性轴索损伤 (DAI)后神经行为的变化和环孢霉素 A(Cs A)的治疗作用。方法 成年 SD大鼠 2 4只 ,随机分为正常对照组、损伤对照组和环孢霉素 A治疗组 (n=8) ,以 Morris水迷宫实验和避暗实验测定伤后不同时间点 DAI大鼠学习记忆能力的变化 ,研究 Cs A的治疗作用。结果 水迷宫定位航行试验发现 ,随训练次数的增多 ,3组大鼠潜伏期 (找到平台所需时间 )总体上呈逐渐下降的趋势 ,但Cs A治疗组潜伏期较损伤对照组缩短 (F=6 0 .4 4 ,P<0 .0 1)。水迷宫空间搜索实验证实 ,Cs A治疗组大鼠平台搜寻成绩好于损伤对照组 ,与正常对照组无差别。避暗实验示正常对照组大鼠潜伏期为 (2 92 .88± 12 .6 1) s,DAI大鼠经 Cs A治疗后 ,进入暗室潜伏期则从 (15 3.2 5± 6 9.36 ) s延长至 (2 2 4 .75± 5 5 .95 ) s(F =71.5 0 ,P<0 .0 5 )。结论  DAI发生后 ,大鼠的学习记忆能力有不同程度下降 ,Cs A治疗可使损伤大鼠的学习记忆能力明显提高。

关 键 词:弥漫性轴索损伤  环孢霉素A  Morris水迷宫
文章编号:1003-0603(2004)04-0214-04
修稿时间:2003-11-01

Neurobehavior changes of rats after diffuse axonal injury and the treatment effect with cyclosporin A
YIN Weidong,XU Bainan,WANG Xiaogang. Neurobehavior changes of rats after diffuse axonal injury and the treatment effect with cyclosporin A[J]. Chinese critical care medicine, 2004, 16(4): 214-217
Authors:YIN Weidong  XU Bainan  WANG Xiaogang
Affiliation:Department of Neurosurgery, General Navy Hospital of PLA, Beijing 100037, China.
Abstract:OBJECTIVE: To observe rat's memory and learning behavior after diffuse axonal injury (DAI) and evaluate the treatment efficacy of cyclosporin A (CsA). METHODS: Twenty-four SD rats were divided into three groups: non-injured group (n=8), vehicle-treated group (n=8) to whom saline was applied and CsA-treated group (n=8). By analyzing behavior changes of rats in Morris water maze and dark-avoidance test, the CsA treatment efficacy was evaluated. RESULTS: The Morris water maze was used to observe rats' memory and learning behavior. During a place-training experiment, the latency to find platform in each trial descended remarkably. Vehicle-treated group exhibited acquisition deficits compared with CsA-treatment group. The latency in each trial was longer in vehicle-treated group (F=60.44, P<0.01). With continued training, the rats' performance in CsA-treatment group was better than those in control brain-injured group in crossing platform test (F=-4.00, P<0.01), but no difference with the normal group. In dark-avoidance test, the rats in normal control group had remembered the electricity shock very fast, and seldom entered into dark chamber. The mean latency time was (292.88+/-12.61) seconds. The latency time of rats in CsA-treated group was (224.75+/-55.95) seconds. The latency time of rats in vehicle-treated group was (153.25+/-69.36) seconds. The time of rats in CsA-treated group was shorter than the time of vehicle-treated group animal(F=-71.50, P<0.05). CONCLUSION: The rats' memory function is impaired after DAI. The learning acquisition and memory retention impairments are ameliorated after administration of CsA.
Keywords:diffuse axonal injury  cyclosporin A  Morris water maze
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