| 大鼠出血性脑水肿水通道蛋白4表达的研究 |
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| 引用本文： | 李燕华,孙善全.大鼠出血性脑水肿水通道蛋白4表达的研究[J].中国危重病急救医学,2003,15(9):538-541. |
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| 作者姓名： | 李燕华 孙善全 |
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| 作者单位： | 重庆医科大学神经生物学研究室,重庆,400016 |
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| 基金项目： | 国家自然科学基金资助项目 ( 30 0 70 2 4 7) |
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| 摘 要： | 目的 :探讨大鼠脑出血后血肿周围水通道蛋白 4(AQP4)的表达变化。方法 :采用定量胶原酶注入大鼠尾状核建立脑出血模型 ,用干湿重法和免疫组织化学法分别检测脑出血后不同时间段脑含水量和 AQP4蛋白的表达。结果 :脑出血后 6h,脑含水量和血肿周围 AQP4蛋白表达增加 ;出血后 72 h达到高峰 ,出血 1周后仍高于正常 ;AQP4蛋白的表达和脑含水量呈显著正相关 (r=0 .985 7,P<0 .0 1)。结论 :AQP4与出血性脑水肿的形成密切相关
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| 关 键 词： | 脑出血 脑水肿 水通道蛋白-4 |
| 文章编号： | 1003-0603(2003)09-0538-04 |
| 修稿时间： | 2003年3月18日 |
Expression of aquaporin-4 protein in brain from rats with hemorrhagic edema |
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| LI Yanhua,SUN Shanquan.Expression of aquaporin-4 protein in brain from rats with hemorrhagic edema[J].Chinese Critical Care Medicine,2003,15(9):538-541. |
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| Authors: | LI Yanhua SUN Shanquan |
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| Institution: | Department of Neurobiology, Chongqing University of Medical Science, Chongqing 400016, China. |
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| Abstract: | Objective: To study the expression of aquaporin4(AQP4) protein in perihematomal tissue after intracerebral hemorrhage(ICH) in rats. Methods: The experimental ICH m odel was established in rats by injecting quantitative collagenase into the left caudate nuclei of the rats. The changes of brain water content(BWC) were measur e d by the wet and dry weight methods. Immunohistochemistry was used to determine the change of expression of AQP4 protein during different periods after ICH. Results: BWC and AQP4 protein expression in perihematomal tissue were increased at 6 ho urs after ICH and reached peak at 72 hours. In one week following ICH, it was st il l higher than normal level. In addition, the expression of AQP4 was positively c orrelated with BWC(r=0 985 7,P<0 01). Conclusion: AQP4 has a relevant relationship with the formation of brain edema after ICH. |
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| Keywords: | intracerebral hemorrhage brain edema aquaporin4 |
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