miR-200a和YAP1在肺癌的不同表达及病理学意义

目的探讨非小细胞肺癌(NSCLC)中miR-200a与Yes相关蛋白1(YAP1)表达量在癌组织和癌旁组织的相关性及其临床病理意义。方法回顾性选取259例非小细胞肺癌患者术后切除的肿瘤组织及癌旁组织为本次研究对象,采用RT-PCR方法检测并比较患者的miR-200a与YAP1表达量在癌组织和癌旁组织的相关性及其临床病理意义。结果所选患者中miR-200a及YAP1在非小细胞肺癌中表达量显示,miR-200a与YAP1的表达量在病理类型、浸润深度、肿瘤分级、肿瘤数目中的比较,差异无统计学意义(P>0.05)。miR-200a与YAP1的表达量在肿瘤分化程度、淋巴结转移的比较中,差异有统计学意义(P<0.05)。所选患者中miR-200a、YAP1在非小细胞肺癌组织表达量比癌旁组织较高,其中,YAP1的非小细胞肺癌组织表达量水平明显高于miR-200a的表达量水平,差异有统计学意义(P<0.05)。miR-200a、YAP1对非小细胞肺癌ROC曲线显示,miR-200a的ROC曲线下面积0.689比YAP1的0.666大,差异有统计学意义(P<0.05)。结论 miR-200a、YAP1表达量水平与非小细胞肺癌临床病理严重程度呈正相关,对非小细胞肺癌诊断有一定预测价值。

Differentiate expression and pathological significance of miR-200a and YAP1 in lung cancer

Objective To investigate the correlation between the expression of miR-200 a and Yesassociated protein 1(YAP1)in non-small cell lung cancer(NSCLC)and its clinicopathological significance.Methods A total of 259 non-small cell lung cancer patients were retrospectively enrolled in this study. The tumor tissues and adjacent tissues were collected. RT-PCR was used to detect and compare the expression of miR-200 a and YAP1 in cancer tissues and adjacent tissues. The relevance and its clinicopathological significance was analyzed. Results There was no significant difference of the expression miR-200 a and YAP1 levels in pathological type,depth of invasion,tumor grade,and number of tumors(P>0.05). The expression levels of miR-200 a and YAP1 were significantly different in the comparison of tumor differentiation and lymph node metastasis(P<0.05). The expression levels of miR-200 a and YAP1 in non-small cell lung cancer tissues were higher than those in adjacent tissues. Among them,the expression level of YAP1 in non-small cell lung cancer tissues was significantly higher than that of miR-200 a. The difference was statistically significant(P<0.05).The area under the ROC curve of miR-200 a(0.689)was larger than that of YAP1(0.666),and the difference was statistically significant(P<0.05). Conclusion The expression levels of miR-200 a and YAP1 are positively correlated with the clinicopathological severity of non-small cell lung cancer and have certain predictive value for the diagnosis of non-small cell lung cancer.