两种采样方法对环境中微生物采样效果的比较研究

摘要 目的 比较棉签拭子与接触碟(Rodac)对不同硬质表面微生物、织物、不同环境(实验室、医院)现场微生物以及人工染菌物体表面的采样效果，为现场微生物采集方法选择提供科学参考。方法 采用棉拭子采样倾注培养法和Rodac接触碟压印法分别对环境中微生物进行采集。结果 棉拭子对织物表面的微生物捕获量高于Rodac接触碟；Rodac接触碟对较洁净的硬质表面微生物捕获量高于棉拭子。Rodac接触碟对人工污染的金黄色葡萄球菌和大肠杆菌捕获量均高于棉拭子。结论 两种采样方法对不同表面污染的微生物捕获量不同，对不同微生物种类捕获量不同。

Comparative study on the effect of two sampling methods on microbial sampling in environment

Objective cial contamination surface between contton swab and Rodac contact dish sampling effect,and to provide scientific reference for the selection of on-site microbial collection methods. Methods cotton swab sampling and pouring culture and Rodac contact plate embossing were used to collect microorganism in the environment. Results The microbial catch of cotton swab on the surface of the fabric was higher than that of Rodac contact dish,and the microbial catch of Rodac contact dish on the clean hard surface was higher than that of cotton swab. The Rodac contact plate caught more live staphylococcus aureus and E. coli than cotton swabs. Conclusion The two sampling methods have different yeilds of microorganisms with different surface contamination and different species of organisms.