青春双歧杆菌和嗜酸乳杆菌对小鼠急性实验性结肠炎的治疗作用

目的通过建立葡聚糖硫酸钠（DSS）诱导的急性期溃疡性结肠炎（UC）小鼠模型,探讨青春双歧杆菌和嗜酸乳杆菌诱导UC缓解的作用。方法 6～8周龄雌性BABL/c小鼠50只,体质量（20.0±2.0）g,随机分为5组：正常对照组（N-7组）、嗜酸乳杆菌（L-7组）、青春双歧杆菌组（B-7组）、柳氮磺胺吡啶（SASP）组（S-7组）和生理盐水对照组（NS-7组）,每组10只。除正常对照组外,采用自由饮用2.5%DSS（分子量3.6万～5万）7天建立小鼠UC模型,且从实验第1天开始,给予青春双歧杆菌、嗜酸乳杆菌（2×10^8 CFU/10 g）和SASP（15 mg/10 g）灌肠治疗7天,观察小鼠一般情况、粪便隐血,计算疾病活动指数（DAI）;实验结束时处死动物,测量全段结肠长度,行组织病理学检查并进行组织学评分。结果青春双歧杆菌、嗜酸乳酸杆均能诱导实验性结肠炎的缓解,其DAI积分、病理组织学评分均低于对照组（P〈0.05）;嗜酸乳酸杆的保护作用强于青春双歧杆菌和SASP。结论青春双歧杆菌和嗜酸乳酸杆菌能有效地减轻急性期UC炎症,且嗜酸乳酸杆菌更有利于诱导急性期UC的缓解。

The therapeutic effect of B. adolescentis and L.acidophilus on acute ulcerative colitis (UC) in mouse model

Objective To establish DSS-induced acute ulcerative colitis（UC） mouse models and then to study the effect of B.adolescentis and L.acidophilus on UC mouse models.Methods Altogether 50 specific pathogen free,20 ± 2 g,6-8 week-old BALB/c female mice were randomly allocated into 5 groups： normal control group（N-7）,L.acidophilus-treated group（L-7）,B.adolescentis-treated group（B-7）,sulfasalazine（salazosulfapyridine,SASP）-treated group（S-7） and NS control group（NS-7）.With the exception of normal control mice,40 BABL/c mice were induced by 2.5% DSS water for 7 days free drinking.These mice were given with 0.2ml B.adolescentis/L.acidophilus/SASP/NS via gavage once a day for 7 days,while normal controls let untreated.These animals were recorded daily both in weight,fecal trait and occult blood（o-toluidine）,and then disease active index（DAI） was accounted.Animals were killed on 8th day morning.Whole colon（from colo-cecal junction to anus） was isolated for examination of length and pieces for hematoxylin and eosin staining,and histological scores were measured.Results B.adolescentis and L.acidophilus could relieve pathogenetic condition of DSS-induced mice.The DAI and histological scores of mice treated with probiotics were much better than those did not treat.The effect of mucosa protection was better in L.acidophilus treated mice.Conclusion The B.adolescentis and L.acidophilus can reduce the inflammation of active UC,and L.acidophilus has more benefits in inducing the relief of active UC.