β-纤维蛋白原-455G/A基因多态性与新疆哈萨克族冠心病的关系

目的分析β纤维蛋白原(FGB)基因启动子区-455 bp位点多态性与新疆哈萨克族冠心病(CHD)的关系。方法采用聚合酶链式反应-限制性片段长度多态性分析法检测新疆哈萨克族220例CHD患者和200例正常对照者的FGB基因启动子区-455 bp位点多态性,并测定血浆纤维蛋白原(Fg)水平。结果①CHD组GA/AA基因型及A等位基因含量均高于对照组,差异有统计学意义(P均<0.05);②CHD组各基因型的Fg水平均高于对照组同种基因型,在CHD和对照组中,不同基因型Fg水平,以AA基因型为最高,GG基因型为最低,组间比较差异均有统计学意义(P<0.05)。结论β纤维蛋白原基因启动子区-455 bp位点多态性与新疆哈萨克族CHD有关,A等位基因是CHD的易感危险因素,可能通过影响Fg水平与CHD产生联系。

The relationship of β-fibrinogen-455 G/A polymorphism with coronary heart disease in Xinjiang kazakans

Objective To explore the association of-455G/A polymorphisms of β-fibrinogen gene with coronary heart disease in Kazakans of Xinjiang.Methods 220 coronary heart disease patients and 200 normal people were involved into this study.-455 G/A polymorphisms of β-fibrinogen gene was analyzed by polymerase chain reaction-restriction fragment length polymorphism（PCR-PFLP）.The plasma fibrinogen also was determined.Results（1） The frequency of GA/AA genotype and A-Allele was significantly higher in coronary heart disease group than that of the control group（P 0.05）.（2） The level of fibrinogen of each genotype in coronary heart disease group was significantly higher than the same genotype of the control group.In each group,the level of fibrinogen of patients with GA genotype was significantly higher than those of patients with GG genotype.The level of fibrinogen of patients with AA genotype was significantly higher than those of patients with GA genotype（P 0.05）.Conclusion β-fibrinogen gene-455 G/A polymorphism may be related to coronary heart disease in Kazakans of Xinjiang.The A-Allele may be the risk genetic factors for coronary heart disease,and may contact with coronary heart disease by influencing the level of fibrinogen.