首页 | 本学科首页   官方微博 | 高级检索  
检索        

8种国产HBsAg试剂盒检测变异HBsAg的效果评价
引用本文:田拥军,覃莉,刘慎沛,雷延昌,赵西平,陆蒙吉,杨东亮.8种国产HBsAg试剂盒检测变异HBsAg的效果评价[J].临床检验杂志,2007,25(4):250-253.
作者姓名:田拥军  覃莉  刘慎沛  雷延昌  赵西平  陆蒙吉  杨东亮
作者单位:1. 华中科技大学同济医学院附属同济医院临床免疫研究室,武汉,430030;华中科技大学同济医学院附属同济医院实验医学研究中心,武汉,430030
2. 华中科技大学同济医学院附属同济医院临床免疫研究室,武汉,430030
3. 华中科技大学同济医学院附属同济医院实验医学研究中心,武汉,430030
4. 华中科技大学同济医学院附属同济医院同济医学院病原生物学系,武汉,430030
基金项目:国家十五科技攻关项目(2001BA705805);湖北省科技攻关项目(2006AA301851-1).
摘    要:目的:评价国内8种HBsAg诊断试剂检测体外表达的18种变异HBsAg的效果。方法:用8种ELISA试剂检测真核细胞表达的18种变异HBsAg的免疫反应性。结果:8种试剂盒检测变异HBsAg的能力不同,漏检率为16.7%~44.4%。K122I、T123N、C124R和G145R变异的HBsAg漏检率最高。结论:8种HBsAg检测试剂盒都不能很好地检出部分变异HBsAg,应改进试剂性能,提高对变异HBsAg的检出率。

关 键 词:乙型肝炎表面抗原  变异  ELISA
文章编号:1001-764X(2007)04-0250-04
收稿时间:2006/12/8 0:00:00
修稿时间:2006-12-082007-03-02

Evaluation of 8 diagnostic kits for detection of variant hepatitis B virus surface antigens expressed in vitro
TIAN Yonjun,QIN Li,LIU Shenpei,LEI Yanehang,ZHAO Xiping,LU Menji,Yang Dongliang.Evaluation of 8 diagnostic kits for detection of variant hepatitis B virus surface antigens expressed in vitro[J].Chinese Journal of Clinical Laboratory Science,2007,25(4):250-253.
Authors:TIAN Yonjun  QIN Li  LIU Shenpei  LEI Yanehang  ZHAO Xiping  LU Menji  Yang Dongliang
Institution:1. Divisionof Clinical Immunology,2. Experimental Research Center, Tongji Hospital of Tongji Medical College ,3. Department of Pathogenic Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
Abstract:Objective To evaluate 8 commercial kits for diagnostic detection of 18 variant hepatitis B virus surface antigens(HBsAg).Methods Eighteen different HBsAg variants were produced in a mammalian expression system and their immunoreactivity was detected by 8 HBsAg diagnostic kits.Results The capacity of these kits for detection of HBsAg variants was quite different.Of the analyzed 18 variants 22.2% to 44.4% of samples were undetectable by one of the commercial HBsAg diagnostic kits.The amino acid substitution of K/R122I,R122K,T123N,C124R and G145R markedly affected the antigen structure of HBsAg resulting misdetection.Conclusion All the 8 commercial kits can not detect the total HBsAg variants,so the capacity of the current HBsAg kits for detection of HBsAg variants should be extended.
Keywords:hepatitis B surface antigen(HBsAg)  variant  ELISA
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《临床检验杂志》浏览原始摘要信息
点击此处可从《临床检验杂志》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号