| 定量检测血浆RASSF1A甲基化在晚期肺癌患者个体化治疗中的应用 |
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| 引用本文： | 陆雅春,潘世扬,王芳.定量检测血浆RASSF1A甲基化在晚期肺癌患者个体化治疗中的应用[J].临床检验杂志,2011,29(4):298-300. |
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| 作者姓名： | 陆雅春 潘世扬 王芳 |
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| 作者单位： | 南京医科大学第一附属医院医学检验科，南京210029;南京医科大学第一附属医院医学检验科，南京210029;南京医科大学第一附属医院医学检验科，南京210029 |
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| 基金项目： | 国家自然科学基金(30972821,30901344,30901262)；江苏省实验诊断学重点实验室基金(XK200731)。 |
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| 摘 要： | 摘要:目的：定量检测晚期肺癌患者化疗过程中血浆RAS相关区域家族1A基因(RASSF1A)甲基化水平，探讨其在个体化治疗中的应用价值。 方法：采集24例晚期肺癌患者化疗前后静脉血共135份，用荧光定量甲基化特异性PCR (MSP)检测RASSF1A基因的甲基化水平，观察化疗前后的变化情况。 结果：13例部分缓解组(partial remission,PR)患者化疗后甲基化定量水平明显升高(P＜0.05)，而11例未缓解组(stable disease,SD或progressive disease,PD)的患者化疗前后甲基化水平无明显变化；生存时间≥12个月的13例患者化疗后甲基化定量水平也明显升高(P＜0.01)，而生存时间<12个月的11例患者化疗前后甲基化水平无明显变化。生存曲线分析显示，化疗后有甲基化水平升高改变的患者总体生存时间更长(P＜0.01）。 结论：晚期肺癌患者化疗前后血浆RASSF1A甲基化定量水平的检测，对评估化疗的疗效及预后有一定的价值，对个体化化疗的药物选择有一定的指导意义。
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| 关 键 词： | 晚期肺癌 甲基化 个体化治疗 RAS相关区域家族1A基因 |
| 收稿时间： | 2011/3/10 0:00:00 |
| 修稿时间： | 2011/4/11 0:00:00 |
Application of quantitative detection of RASSF1A methylation in plasma for personalized chemotherapy of patients with advanced lung cancer |
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| LU Ya-chun,PAN Shi-yang,WANG Fang,XIA Wen-ying,XIE Er-fu,SUN Rui-hong,GAO Li,CHEN Dan,ZHANG Li-xia,HUANG Pei-jun,XU Ting,GENG Yan,PENG Ying,QIN Xue-jun.Application of quantitative detection of RASSF1A methylation in plasma for personalized chemotherapy of patients with advanced lung cancer[J].Chinese Journal of Clinical Laboratory Science,2011,29(4):298-300. |
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| Authors: | LU Ya-chun PAN Shi-yang WANG Fang XIA Wen-ying XIE Er-fu SUN Rui-hong GAO Li CHEN Dan ZHANG Li-xia HUANG Pei-jun XU Ting GENG Yan PENG Ying QIN Xue-jun |
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| Institution: | LU Ya-chun,PAN Shi-yang,WANG Fang,XIA Wen-ying,XIE Er-fu,SUN Rui-hong,GAO Li,CHEN Dan,ZHANG Li-xia,HUANG Pei-jun,XU Ting,GENG Yan,PENG Ying,QIN Xue-jun(Department of Laboratory Medicine,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,Jiangsu,China) |
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| Abstract: | Objective To quantitatively detect methylation level of Ras association domain family 1A gene(RASSF1A) in plasma of patients with advanced lung cancer and explore the application value in personalized chemotherapy.Methods A total of 135 blood samples were collected from 24 lung cancer patients before chemotherapy and after every therapeutic cycle.The methylation level of RASSF1A gene in plasma was detected by real-time methylation specific PCR,and the changes of methylation level before and after chemothera... |
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| Keywords: | advanced lung cancer methylation personalized chemotherapy Ras association domain family 1A gene |
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