血管内栓塞大脑中动脉制作非人灵长类卒中慢性期模型的研究

目的建立非人灵长类脑梗死慢性期动物模型。方法雄性成年恒河猴10 只，血管内介入栓塞动物利手对侧大脑中动脉。依栓塞节段不同，分为M1 段栓塞组(n=3)、上干栓塞组(n=5)和下干栓塞组(n=2)。通过标准神经量表和小槽食丸取回任务评定运动功能。结果M1 段栓塞组均在急性期死亡。上干栓塞组均存活，MRI 可见梗死灶，急性期一侧肢体瘫痪，其中4 只功能障碍在慢性期持续存在，另外1 只运动功能恢复。下干栓塞组急性期一侧肢体瘫痪，运动缺陷恢复迅速且完全。结论大脑中动脉上干栓塞动物模型存活率高，慢性期存在运动功能障碍，适合于慢性期研究。

Nonhuman Primate Chronic Stroke Model with Middle Cerebral Artery Endovascular Embolism

Objective To establish chronic cerebral infarction animal model in nonhuman primate.Methods 10 adult male rhesus monkeys were embolized the middle cerebral artery(MCA) in contra-lateral of handedness,and divided into M1 segment embolism group(n= 3),upper trunk embolism group(n=5),and lower trunk embolism group(n=2).Acute neurological deficit was evaluated with standard neurological scale,and the motor function in chronic stage was assessed with a task of retrieving food pill in wells.Results Animals in M1 segment embolism group all died 38~62 h after surgery.Upper trunk embolism group survived,and MRI showed front parietal cortex infarction contra-lateral paralyzed side.All of them paralyzed one side in acute stage,and 4 of them persisted dysfunction in chronic stage,that could not finish the task of retrieving food pill in wells;only one completed the task.The lower trunk embolism group paralyzed one side in acute stage,but recovered quickly and completely,that finished the task within 7 d.Conclusion Embolism of MCA upper trunk can cause infarction of precise and proper size with one side limb dysfunction in the acute stage and long-term dysfunction in most animals,which is feasible for treatment and neural plasticity research in recovery.