脑源性神经营养因子在孤独症模型鼠颞叶皮层中的表达

目的研究颞叶皮层脑源性神经营养因子(BDNF)表达与孤独症的关系。方法孕12.5 d Wistar 大鼠腹腔注射丙戊酸钠(VPA) 600 mg/kg，观察仔鼠(VPA组)的行为学特征，应用免疫组化法检测VPA组与对照仔鼠(对照组，腹腔注射等量生理盐水)颞叶皮层BDNF 表达。结果与对照组相比，VPA 组表现为低体重(P<0.05)，睁眼时间延迟(P<0.05)，协调性反应差(P<0.05)，方向趋向性反应迟缓(P<0.05)；社交行为次数减少(P<0.05)、潜伏期延长(P<0.05)、持续时间缩短(P<0.05)，重复行为增多(P<0.05)。小脑浦肯野细胞数量减少。出生后1 d、7 d、14 d 时，VPA 组颞叶皮层BDNF 表达明显高于对照组(P<0.01)，而出生后35 d、49 d 时，VPA组颞叶皮层BDNF 表达明显低于对照组(P<0.01)。结论颞叶BDNF 表达参与孤独症的发病过程。

Expression of Brain-derived Neurotrophic Factor in Temporal Cortex of Autism Model Rats

Objective To explore the role of brain-derived neurotrophic factor(BDNF) in pathogenesis of autism.Methods 12.5 d pregnant Wistar rats were injected sodium valproate(VPA) 600 mg/kg or normal saline(NS).Their newborn rats were observed ethologically.The expression of BDNF was measured in their temporal cortex with immunohistochemical stain.Results Compared with NS group,VPA group expressed less body mass(P<0.05),eyes opening delay(P<0.05),poorer coordination response(P<0.05),slower taxis response(P< 0.05).The number of social behavior decreased(P<0.05),latency increased(P<0.05),duration shortened(P<0.05),repetitive activities increased(P<0.05).Purkinje cells reduced in cerebellum.The expression of BDNF increased significantly in temporal cortex 1 d,7 d and 14 d postnatally(P<0.01),but decreased 35 d and 49 d postnatally(P<0.01).Conclusion BDNF plays a role in the pathogenesis of autism.