电针对血管性痴呆大鼠脑白质纤维和学习记忆功能的效果

目的 观察电针百会、神庭对血管性痴呆(VD)大鼠脑白质纤维和学习记忆能力的影响。 方法 Sprague-Dawley大鼠32只分离双侧颈总动脉,假手术组(n = 8)不结扎,其余造模成功后随机分为模型组(n = 8)、非穴组(n = 8)和电针组(n = 8)。电针组电针百会和神庭穴,非穴组刺激腋下非经非穴点,每天1次,共28 d。干预前后采用新物体识别试验进行评定,弥散张量成像观察大鼠脑白质纤维。 结果 干预前,与假手术组相比,模型组、非穴组和电针组新物体偏爱系数降低(P< 0.05),后三组间无显著性差异(P> 0.05);干预后,电针组新物体偏爱系数较模型组增高(P < 0.05)。干预前,与假手术组相比,模型组、非穴组和电针组胼胝体、扣带回、海马白质纤维各向异性分数(FA)降低;干预后,电针组海马、扣带回、胼胝体、外囊白质纤维FA较模型组增加。 结论 电针百会、神庭能改善VD大鼠记忆功能,可能与前额叶皮质、海马等脑区白质纤维修复有关。

Effects of Electroacupuncture on White Matter Fibers and Learning-memory Function in Rats with Vascular Dementia

Objective To observe the effect of electroacupuncture at Baihui(DU20)and Shenting(DU24)acupoints on white matter fiber and learning-memory function in rats with vascular dementia(VD).Methods Sprague-Dawley rats were randomly divided into operation group and sham group(n=8).The operation group accepted two-vessel occlusion,while the sham group only separated the bilateral common carotid arteries without ligation.The rats modeled successfully were randomly divided into model group(n=8),non-acupoint group(n=8)and electroacupuncture group(n=8).The electroacupuncture group accepted electroacupuncture at Baihui and Shenting,and the non-acupoint group accepted electroacupuncture at axillary non-acupoint,once a day for 28 days.All the rats were tested with object recognition test before and after intervention,while the white matter fibers were observed with Diffusion Tensor Imaging.Results Compared with the sham group,the preference coefficients of the model group,the non-acupoint group and the electroacupuncture group decreased before intervention(P<0.05),and there was no significant difference among the later three groups(P>0.05).The preference coefficients increased in the electroacupuncture group compared with the model group after intervention(P<0.05).The fractional anisotropy(FA)of corpus callosum,cingulate gyrus and hippocampus decreased in the model group,the non-acupoint group and the electroacupuncture group compared with the sham group before intervention.FA of hippocampus,cingulate gyrus,corpus callosum and external capsule increased in the electroacupuncture group compared with the model group after intervention.Conclusion Electroacupuncture at Baihui and Shenting can improve learning-memory function of VD rats,which may be related with repairment of white matter fibers in prefrontal cortex,hippocampus and others.