银杏内酯B对神经干细胞分化的影响及其机制研究

目的观察银杏内酯B对体外培养的神经干细胞（NSCs）分化的影响。方法用含不同浓度银杏内酯B（20mg／L、40mg／L、60mg／L）的分化培养基培养NSCs 3d和7d，测量细胞突起长度和胞体面积，用免疫荧光检测神经丝蛋白-200（NF-200）、胶质纤维酸性蛋白（GFAP）及少突胶质细胞特异性蛋白（CC-1）、细胞因子信号抑制因子2（SOCS2）、DNA结合抑制因子2（Id2）的表达。结果银杏内酯B组细胞突起增长，胞体增大；NF阳性神经元样细胞百分率增加，GFAP阳性星形胶质样细胞百分率随银杏内酯B浓度增加而增加；SOCS2阳性细胞百分率（35．46％）较对照组（22．17％）明显增加（P〈0．01），银杏内酯B组Id2阳性细胞百分率（51．52％）较对照组（66．24％）明显减少（P〈0．01）。结论银杏内酯B可促进分化细胞成熟，促进NSCs向神经元分化；星形胶质细胞百分率的增高与银杏内酯B呈剂量依赖关系。

Mechanisms and Effect of Ginkgolide B on the Differentiation of Neuron Stem Cells

Objective To observe the effect of Ginkgolide B of various consistency on the differentiation of neuron stem cells (NSCs).MethodsNSCs were cultured in differentiation medium containing Ginkgolide B of various consistency for 3 and 7 days, the neurites length and cell body area were measured by inverted phase-contrast micrograph, then neurofilament-200 (NF-200), glial fibrillary acidic protein (GFAP), adenomatus polyposis coli (CC-1) expression were detected and counted by fluorescence microscope. The suppressor of cytokine signaling-2 (SOCS2), inhibitor of DNA binding-2 (Id2) were alsoimmunostained. The percentage of positive cells were counted respectively.ResultsThe neurites length and cell body area in Ginkgolide B groups were obviously larger than that in the control group. The percentage of NF, GFAP positive cells in Ginkgolide B groups increased with dosage increasing of Ginkgolide B. Compared with the normal control group, the percentage of SOCS2 positive cells increased significantly ( P<0.01) and the percentage of Id2 positive cells decreased significantly ( P<0.01) in Ginkgolide B groups.ConclusionGinkgolide B can promote NSCs to differentiate into neuron and astrocyte, the percentage of astrocyte is increased with a dosage-dependent relationship with Ginkgolide B.