PARP1单核苷酸多态性对晚期胃癌奥沙利铂化疗反应及预后的影响

目的 探讨多聚腺苷二磷酸核糖聚合酶(PARP-1)单核苷酸多态性(SNP)与晚期胃癌患者化疗反应及生存时间的关联性.方法 选择2016年5月至2019年12月于苏州科技城医院肿瘤内科收治的161例晚期胃癌患者,给予奥沙利铂联合替吉奥化疗,化疗前抽空腹静脉血5 ml用于提取基因组DNA.TaqMan探针法鉴别PARP1基...

Impacts of PARP1 single nucleotide polymorphism on response to oxaliplatin-based chemotherapy and prognosis of advanced gastric cancer

Objective To investigate the associations of single nucleotide polymorphism(SNP)in poly-adenosine diphosphate-ribose polymerase(PARP)-1 gene with chemotherapy response and survival time in patients with advanced gastric cancer.Methods From May 2016 to December 2019,161 patients with advanced gastric cancer in Suzhou Science and Technology Town Hospital were treated with oxaliplatin and teggio chemotherapy.Before treatment,5 ml of fasting venous blood was drawn to extract genomic DNA in all patients.TaqMan probe was used to identify the genotypes of rs907187C/G,rs1805414T/C and rs1136410T/C polymorphic loci of PARP1 gene,and the influences of these polymorphisms on objective response rate(ORR)and overall survival(OS)were analyzed.Results The rs907187C/G polymorphism was significantly associated with chemotherapy sensitivity.The ORR to chemotherapy was significantly higher in the carriers of G variant allele,and CC,CG and GG genotypes were 23.2%,42.2%and 53.7%respectively(P=0.007).The ORR to chemotherapy of heterozygous CG genotype was 2.362 times that of CC genotype(P=0.035).The ORR of variant homozygous GG genotype was 3.812 times that of CC genotype(P=0.003).The ORR of CG and GG genotype was 2.854 times that of CC genotype(P=0.005).Kaplan-Meier analysis showed that rs1136410T/C polymorphism was significantly associated with OS;the median OS of the carriers of C variant allele was significantly prolonged and were 10.0,13.1 and 16.9 months,respectively in TT,TC and CC genotype(P=0.006);the median OS of TC and CC genotype was 14.5 months and was significantly longer than that of TT genotype(P=0.004).Cox proportional hazards model analysis showed that rs1136410T/C polymorphism was an independent influencing factor of OS in patients with advanced gastric cancer(P=0.010,OR=1.667,95%CI:1.131-2.458).No significant associations of rs1805414T/C polymorphism with ORR to chemotherapy and prognosis were found.Conclusion The rs907187C/G polymorphism of PARP1 gene can affect the ORR of chemotherapy,and rs1136410T/C polymorphism may be a predictor of survival time in patients with advanced gastric cancer.