脑膜瘤患者IGF-1、IGFBP-3测定的意义

目的：探索人脑膜瘤患者IGF-1、IGFBP-3与脑膜瘤发生发展的关系，为提高脑膜瘤的早期诊断率提供实验依据。方法正常对照组、脑膜瘤组各30例，血清标本均用免疫放射分析法测定IGF-1、IGFBP-3浓度。术中分别取两组的组织标本，行常规HE切片染色及IGF-1免疫组化染色，观察免疫组化染色结果。结果脑膜瘤组血清IGF-1水平明显升高，与对照组比较差异有统计学意义（P＜0.05）。脑膜瘤组血清IGFBP-3水平也明显升高（P＜0.05）。二组血清IGF-1水平均与血清IGFBP-3水平呈正相关（r=0.594、0.474，P＜0.05）。正常脑组织中IGF-1无表达；脑膜瘤组织中的IGF-1表达水平也升高（P＜0.05）。结论IGF-1可能促进了脑膜瘤的发生发展。IGFBP-3可能协同IGF-1促进了脑膜瘤的发生发展。

The Signiifcance of Determination of Insulin-like Growth Factor-1 and Insulin-like Growth Factor Binding Protein-3 in Meningioma Patients

Objective To determine expression of IGF-1 in tumor tissues and serum IGF-1, IGFBP-3 levels in meningioma patients, and to investigate both relationships and to discuss the exact roles of IGF-1 and IGFBP-3 in development of meningioma in order to diagnose early meningioma by detecting serum IGF-1, IGFBP-3 levels. Methods The patients were divided into two groups （healthy control group and meningioma group）, each group including 30 cases. A 4 mL blood sample from each fasting case was drawn in the early morning, which was sepa-rated by centrifugation immediately and was stored under-20℃. Serum IGF-1 and IGFBP-3 levels were deter-mined by immunoradiometric assays （IRMA）. Tissue samples in the operation were made in routine HE staining and IGF-1 immunohistochemical staining. Staining results were watched with microscope. Results （1）Serum IGF-1 levels in meningioma were also signiifcantly higher than those in healthy control （P〈0.05）;（2）Serum IGFBP-3 levels in meningioma were signiifcantly higher than those in healthy control （P〈0.05）;（3）Serum IGF-1 levels correlated signiifcantly with serum IGFBP-3 levels in two groups （r=0.594, 0.474, P〈0.05）;（4）Expressions of IGF-1 in meningioma were also obviously increased （P〈0.05）. Conclusion （1）IGF-1 improved the development of meningioma;（2）Serum IGF-1 level were probably increased in meningioma patients, which still waited further investigation;（3）Serum IGFBP-3 level were probably increased in meningioma patients;（4）IGFBP-3 probably co-operated with IGF-1 to improved the development of meningioma, which still waited further investigation;（5） Increased serum IGF-1 levels signiifcantly correlated with serum IGFBP-3 in control cases, which suggested there existed some negative feedback mechanism to regulate both serum levels in healthy physiological status.