肾细胞癌端粒酶活性测定的研究

目的研究端粒酶活性与肾细胞癌之间的关系。方法采用改良的TRAP法对肾细胞癌组织和癌旁正常肾组织中端粒酶活性进行检测,结果肾癌组织中和癌旁正常肾组织中端粒酶活性阳性率差异有显著性(P<0．01);高分化组与低分化组端粒酶活性差异无显著性(P>0．05);早期肾癌组与中晚期肾癌组端粒酶活性差异无显著性(P>0．05)。结论肾细胞癌组织中端粒酶活性较正常组织高,肾癌端粒酶活性与其恶性程度无相关性,端粒酶活性测定可能作为肾癌诊断的分子水平标志物。

The Study on Telomerase Activity in Human Renal Cell Carcinoma

Objective To study the telomerase activity in human renal cell carcinoma (RCC) and to evaluate their correlation. Methods The telomerase activity was examined in a modified telomeric repeat amplification protocol (TRAP) in RCC tissues and in the adjacent normal kidney tissue,and assessed for associations with clinical and pathological variables of the disease. Results Mean telomerase activity of RCC was significantly greater than that of normal renal tissue (P<0.01); Telomerase activity in RCC was,however, not associated with pathological grade(P> 0.05),and not associated with clinical stage(P>0.05). Conclusion The positive rate of telomerase activity was significantly higher in RCC tissues.Telomerase might play a crucial role in an initial step of the development of RCC, but was not correlated with the degree of malignancy.The positive rate of telomerase activity might serve as a potential marker for estimating the biologic characteristics of RCC and as a noninvasive biomarker for disease and follow-up.