细胞表面α2,3唾液酸结构与胃癌细胞AGS增殖、迁移的关系及相关基因表达的初步研究

目的 检测6个α2,3唾液酸转移酶基因在胃癌细胞AGS的表达,以及AGS细胞表面的α2,3唾液酸结构对增殖、迁移能力的影响.方法 通过RT-PCR分别检测6个基因在胃癌细胞AGS mRNA水平的表达情况.以不同活性浓度的α2,3唾液酸酶处理AGS细胞,通过MTT法检测其生长曲线的改变,通过划痕试验检测其迁移能力的改变.结果 在胃癌细胞AGS中,α2,3唾液酸转移酶家族的6个成员有两个在mRNA水平表达.分别是ST3Gall和ST3Ga14;α2,3唾液酸酶处理后,AGS生长周期缩短,迁移能力显著增强.结论 AGS细胞表面的α2,3唾液酸结构可能通过影响细胞之间的识别、改变信号通路,延长AGS细胞的生长周期,抑制其迁移能力.

A Preliminary Study on the Effects of Membrane α2,3 Polysialic Acid Residues on Human Gastric Cancer Cell AGS's Growth and Migration and Several Related Genes' Expression

Objective To examine the membrane α 2,3 polysialic acid residues of AGS,and estimate their effects on the growth and migration ability of AGS,and investigate the expression of several a 2,3 linked polysialic acids related genes＇ mRNA-level expression. Methods The expression of six α 2,3 sialyltransferases was evaluated by RT-PCR,the effects of α 2,3 sialidase treatment on the growth and migration ability of AGS were examined by MTT and wound-healing assay. Results Two members of the α 2,3 sialyltransferase family, ST3Gal 1 and ST3GaI4 are expressed in AGS cells, α 2,3 sialidase treatment shortened AGS＇ s growth cycle,significantly increased AGS＇ s migration capacity. Conclusion The membrane α2,3 polysialic acid residues of gastric cell AGS can lengthen the growth cycle of AGS and inhibit its migration ability through cellular recognition and signal transduction.