慢性心理应激对大鼠子宫内膜Hoxa-10表达的影响

目的探讨慢性心理应激对大鼠子宫内膜Hoxa-10表达的影响。方法取40只同日龄的雌性大鼠,观察组与对照组各20只。对照组在实验室群居饲养,观察组按浸笼模型制模。采用免疫组化S-P法,检测大鼠动情周期子宫内膜雌激素受体、孕激素受体及Hoxa-10的表达水平。结果观察组动情间期孕激素受体表达较对照组下调(P0.05)、动情间期Hoxa-10表达较对照组下调(P0.05),Hoxa-10表达和孕激素受体表达呈正相关(直线正相关,r=0.777)。结论慢性心理应激能降低大鼠动情间期Hoxa-10表达,Hoxa-10表达缺陷可能是导致子宫容受性降低的重要原因之一。

Effect of chronic psychological stress on expression of Hoxa-10 gene in endometrium of rats

Objective To explore the effect of chronic psychological stress on expression of Hoxa-10 homoeobox gene in endometrium of rats.Method Divide 40 rats of the same age in days into observation group and control group equally.Rats in control group receive gregarious feeding in lab.Rats in observation group receive cage model.Test the expression level of estrogen receptor,progesterone receptor and Hoxa-10 of endometrium in the physiological cycle by immunohistochemical SP.Result Progesterone receptor in estrus is lower in observation group than in control group（P0.05）.Expression of Hoxa-10 in estrus is lower in observation group（P0.05）.Expression of Hoxa-10 is positively correlated with expression of progesterone receptor（positive linear correlation,r=0.777）.Conclusion Chronic psychological stress can reduce the expression of Hoxa-10 in estrus of rats.Deficiency in expression of Hoxa-10 may be one of the main causes of uterine receptivity reduction.