三维准连续ASL评价兔软组织肿瘤抗血管生成的可行性研究

目的通过病理对照来研究三维准连续性动脉自旋标记(3D-PCASL)用于评价兔软组织VX2肿瘤抗血管生成的可行性。材料与方法 24只新西兰瘤兔大腿下段肌肉内VX2肿瘤接种成功,对实验组12只和对照组12只瘤兔分别采用恩度(endostatin)/盐水比例为1.5 mg/8 ml溶液和0.9%生理盐水8 ml连续瘤周肌注14 d。瘤兔下肢采用3D-PCASL序列进行扫描,3D-PCASL选取肿瘤组织最大层面,分别测量肿瘤边缘和肿瘤中心灌注参数BFASL值,与病理指标微血管密度(MVD)和血管内皮生长因子(VEGF)表达状况进行对照。结果实验组肿瘤边缘及中心区域MVD值分别与BFASL值具有良好的相关性(r=0.891、r=0.626,P0.05),对照组肿瘤中心及边缘治疗后MVD值分别与BFASL值具有良好的相关性(r=0.960、r=0.917,P0.05),均与VEGF的表达情况相对应。结论 BFASL值与MVD值在软组织VX2肿瘤抗血管生成治疗前后具有良好的相关性,3D-PCASL技术用于评价软组织肿瘤抗血管生成治疗是可行的。

The feasibility study of 3D-PCASL evaluating the anti-angiogenesis of soft tissue tumor

Objective:To investigate the feasibility of anti-angiogenesis of VX2 soft tissue tumor by 3D-PCASL and MVD.Materials and Methods:VX2 tumor was inoculated in the muscular tissue of lower section of femur of 24 New Zealand rabbits successfully. The experimental group and the control group contained 12 subjects, respectively injected in muscles around the tumor with Endostar solution (Endostar: saline ratio of 1.5 mg/8 ml) and 0.9% saline 8 ml,for a period of 14 days. BFASL values were respectively measured by arterial spin labeling (ASL) via selecting the tumor maximum level. BFASL values were compared with those parameters of pathology, which including MVD and VEGF.Results:The MVD values of the central and margin region of experimental group showed a positive correlation with BFASL values (r= 0.891,r= 0.626,P<0.05 ), the MVD values of the central and margin region of control group showed a positive correlation with BFASL values (r=0.960,r=0.917, P<0.05),they all corresponded to the expression of VEGF.Conclusions:The BFASL values and the MVD values showed a positive correlation in evaluation of anti-angiogenesis of VX2 soft tissue tumor. The 3D-PCASL might be feasible for evaluation of anti-angiogenesis of soft tissue tumor.