促甲状腺激素受体抗体对人肝癌细胞HepG2凋亡和增殖的影响

目的 格雷夫斯病(Graves disease,GD)患者肝功能损害发病率高,可能与升高的促甲状腺激素受体抗体(TRAb)相关,探讨TRAb对肝细胞损伤的机制.方法 用含不同浓度TRAb的GD患者血清、正常人血清及胎牛血清的DMEM培养基处理人肝癌细胞株HepG2细胞,采用异硫氰酸荧光素(annexin V-FITC)/碘化丙啶(propidiumiodide,PI)及MTT法分别检测细胞的凋亡和增殖状况.结果 5％、10％及20％ TRAb的GD血清能促进HepG2细胞增殖,且HepG2细胞凋亡较对照组增高明显,以中晚期凋亡为著.结论 TRAb能诱导人肝癌细胞HepG2细胞凋亡,参与肝细胞损伤的发生.

Thyrotropin receptor antibody induced-apoptosis and proliferation in human hepatoma HepG2 cells

Objective Patients with Graves disease（GD） have a high prevalence of hepatic dysfunction,which may be related to elevated thyrotropin receptor antibody（TRAb）. The study was designed to investigate the possible mechanism of TRAb induced hepatic dysfunction. Methods Human hepatoma HepG2 ceils were treated with different serum concentrations of GD patients, healthy controls and fatal bovine serum（FBS）. The cell apoptosis and proliferation of HepG2 cells were analyzed by annexin V-FITC/PI and MTT, respectively. Results 5 %, 10% and 20 % serum of GD promoted the proliferation of HepG2. The apoptosis rates of GD serum were higher than those of normal and FBS controls,in which both interim and late apoptosis were significant. Conclusion TRAb can induce apoptosis in HepG2 cells ,which may contribute to the hepatic dysfunction.