首页 | 本学科首页   官方微博 | 高级检索  
检索        

高发区自然人群贲门癌血清蛋白指纹图诊断模型的建立及临床价值
引用本文:于卫芳,张立玮,王士杰,郑树,余捷凯,王顺平,吴明利,郭晓青,高扬.高发区自然人群贲门癌血清蛋白指纹图诊断模型的建立及临床价值[J].临床荟萃,2006,21(12):841-844.
作者姓名:于卫芳  张立玮  王士杰  郑树  余捷凯  王顺平  吴明利  郭晓青  高扬
作者单位:1. 河北医科大学第四医院,内镜室,河北,石家庄,050011
2. 浙江大学肿瘤研究所,浙江,杭州,310009
基金项目:河北省科技计划项目基金资助(No:052761361,No:03276196D-33);河北省重大攻关课题基金资助(No:03276198D)
摘    要:目的 用表面增强激光解吸离子化飞行时间质谱(SELDI-TOF-MS)分析食管癌高发区自然人群中贲门癌和正常对照血清蛋白表达谱的改变,筛选并建立高发区贲门癌血清蛋白指纹图诊断模型并探究其临床价值。方法 采用CMIO蛋白芯片及SELDI-TOF-MS技术对34例贲门癌和38例正常对照者血清蛋白指纹图谱进行检测,所得结果用ZUCI-蛋白芯片数据分析系统(ZUCI-Protein Chip Data Analyze System)软件包分析,建立贲门癌蛋白指纹图诊断模型,并用留-法交叉验证作为评估模型、判别效果的方法。结果通过软件包运算,用3个质荷比峰(5643.45793、8570.82126、15940.1533m/z)建立了贲门癌蛋白指纹图诊断模型,其准确度93.06%,敏感度85.29%,特异度100%,阳性预测值100%。结论 本组建立的诊断模型可以有效区分贲门癌和健康人,为肿瘤高发区贲门癌的诊断与筛查提供了一条崭新途径。

关 键 词:贲门癌  表面增强激光解吸离子化飞行时间质谱  蛋白芯片  诊断模型  留-法交叉验证
文章编号:1004-583X(2006)12-0841-04
收稿时间:2006-03-15
修稿时间:2006-03-152006-04-07

Establishment of diagnostic model of serum protein fingerprint patterns for cardia cancer in high incidence area and its clinical value
YU Wei-fang,ZHANG Li-wei,WANG Shi-jie,ZHENG Shu,YU Jie-kai,WANG Shun-ping,WU Ming-li,GUO Xiao-qing,GAO Yang.Establishment of diagnostic model of serum protein fingerprint patterns for cardia cancer in high incidence area and its clinical value[J].Clinical Focus,2006,21(12):841-844.
Authors:YU Wei-fang  ZHANG Li-wei  WANG Shi-jie  ZHENG Shu  YU Jie-kai  WANG Shun-ping  WU Ming-li  GUO Xiao-qing  GAO Yang
Institution:Department of Endoscopy, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011 ,China
Abstract:Objective To analyze the alterations of serum protein patterns in cardia cancer patients coming from high incidence area of esophageal cancer by surface-enhanced laser desorption/ionization time of flight mass spectrometry(SELDI-TOF-MS),screen and build diagnostic model of cardia cancer in high incidence area and investigate its clinical value.Methods SELDI-TOF-MS and CM10 protein chip were used to detect the serum protein patterns of 34 cases of cardia cancer patients and 38 cases of healthy controls.The data was analyzed and the diagnostic model was established by using ZUCI-protein chip data analyze system software package.The diagnostic model was evaluated and validated by leave one cross validation.Results A diagnostic model consisting of three protein peaks(5 643.457 93,8 570.821 26,15 940.153 3 m/z) could do the best in the diagnosis between cardia cancer and controls.Its accuracy was 93.06%,sensitivity 85.29%,specificity 100%,positive value 100%.Conclusion The diagnostic model formed by three protein peaks can do the best in discriminating cardia cancer from controls.It provides a new approach for diagnosing and screening cardia cancer in high incidence area of esophageal cancer.
Keywords:cardia cancer  surface enhanced laser desorption/ionization time-of-flight mass spectrometry  protein chip  diagnostic model  leave one cross validation
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号