PD-1在急性白血病异基因造血干细胞移植后T淋巴细胞的表达

目的探究PD-1在急性白血病患者异基因造血干细胞移植(allo-HSCT)后外周血T淋巴细胞表达的动态变化及临床意义。方法采集23例健康志愿者(对照组),40例急性白血病患者allo-HSCT(移植组)后1、2、3、4.5、6个月的外周血标本,采用流式细胞术对各标本CD3^(+)CD4^(+)、CD3^(+)CD8^(+)T淋巴细胞程序性死亡蛋白1(PD-1)的表达水平进行监测,分析移植组和对照组的PD-1表达水平差异。结果急性白血病患者allo-HSCT后1、2个月PD-1在CD3^(+)CD4^(+)、CD3^(+)CD8^(+)T淋巴细胞的表达水平高于对照组;3、4.5、6个月PD-1在CD3^(+)CD4^(+)T细胞的表达水平高于对照组,且随移植时间呈现下降趋势,在CD3^(+)CD8^(+)T淋巴细胞的表达水平与对照组差异无明显统计学意义(P均>0.05)。结论急性白血病患者allo-HSCT后早期PD-1在CD3^(+)CD4^(+)T淋巴细胞的表达较正常水平升高且随移植时间呈现下降趋势;在CD3^(+)CD8^(+)T淋巴细胞的表达于移植后早期呈现一过性升高。

Expression of PD-1 on T-lymphocytes in acute leukemia after allogeneic hematopoietic stem cell transplantation

Objective To explore the dynamic change and clinical significance of PD-1(programmed cell death protein 1)expression on T-lymphocytes in acute leukemia after allogeneic hematopoietic stem cell transplantation(allo-HSCT).Methods Twenty-three healthy volunteers(the control group)and 40 patients with acute leukemia(the transplantation group)were selected,peripheral blood samples were collected 1,2,3,4,5,6 months after allo-HSCT,and PD-1 expression levels on CD3⁺CD4⁺and CD3⁺CD8⁺T-lymphocytes were tested by the flow cytometry.The differences of PD-1 expression levels between the allo-HSCT group and the control group were analyzed.Results The expression levels of PD-1 on CD3⁺CD4⁺and CD3⁺CD8⁺T-lymphocytes in acute leukemia patients in the transplantation group at 1 and 2 months after allo-HSCT were higher than those of the control group.The PD-1 expression levels on CD3⁺CD4⁺T-lymphocyte in the transplantation group were higher than those of the control group at 3,4,5 and 6 month after allo-HSCT,but they showed a downtrend with the transplantation time,while the PD-1 expression levels in CD3⁺CD8⁺T-lymphocyte had no obvious statistical significance(P>0.05 for all).Conclusion The PD-1expression levels on CD3⁺CD4⁺T-lymphocyte in patients with acute leukemia after allo-HSCT were higher than those normal levels at the early stage and showed a downtrend with the transplantation time;while the PD-1 expression levels on CD3⁺CD8⁺T-lymphocyte were increased transiently at the early stage after allo-HSCT.