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Determination of naproxen and its desmethyl metabolite in human plasma or serum by high performance liquid chromatography.
Authors:J T Slattery  G Levy
Affiliation:Department of Pharmaceutics, School of Pharmacy, State University of New York at Buffalo, Amherst, N.Y., 14260, U.S.A.
Abstract:1. A liquid chromatographic method has been developed for the simultaneous determination of the anti-inflammatory agent naproxen and its metabolite, 6-0-desmethyl-naproxen, in human plasma or serum. 2. After addition of p-chlorowarfarin as the internal stardard, plasma is acidified and extracted with either chloroform (for assay of naproxen only) or ethyl acetate (for assay of naproxen and desmethyl-naproxen), the organic solvent is evaporated, the residue is dissolved in acetonitrile and injected onto a reverse phase column coupled with an ultra-violet absorbance detector. 3. Drug and metabolite can be detected readily in concentrations above 2 micrograms per ml in 0.5 ml samples. Salicylic acid and its metabolites and a number of other durgs were found not to interfere with the assay.
Keywords:Correspondence. Dr. Gerhard Levy   Department of Pharmaceutics   School of Pharmacy   State University of New York at Buffalo   Amherst   N.Y.   14260   U.S.A.
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