| 超声造影分析二乙基亚硝胺诱发性大鼠肝癌模型的实验研究 |
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| 引用本文： | 范培丽,李正,曹佳颖,夏罕生,王文平.超声造影分析二乙基亚硝胺诱发性大鼠肝癌模型的实验研究[J].中国超声医学杂志,2020(4):356-358. |
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| 作者姓名： | 范培丽 李正 曹佳颖 夏罕生 王文平 |
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| 作者单位： | 复旦大学附属中山医院超声科、上海市影像医学研究所、复旦大学超声医学与工程研究所;复旦大学附属中山医院临床医学研究院 |
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| 基金项目： | 国家自然科学基金(No.81571676);上海市医学领军人才项目。 |
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| 摘 要： | 目的探讨超声造影评估二乙基亚硝胺诱发性大鼠肝癌模型的可行性。方法20只雄性Wistar大鼠采用30mg/kg腹腔注射二乙基亚硝胺。第14周开始每周轮流抽取5只大鼠进行超声检查,发现肝脏结节的大鼠,进行超声造影(CEUS)检查。检查后处死取标本进行病理诊断。结果大鼠超声检查前死亡率45%(9/20),肝癌造模成功率80%(16/20)。肝细胞癌(HCC)病灶超声检出率72.5%(29/40)。55.2%(16/29)HCC病灶呈“高回声增强-无廓清”为主要CEUS增强模式。结论此动物肝癌模型成瘤率较高,CEUS可用于此模型的评估。
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| 关 键 词： | 二乙基亚硝胺 动物模型 肝细胞癌 超声造影 |
Evaluation of Contrast-enhanced Ultrasound for the Rat Hepatocellular Carcinoma Model Induced by Diethylnitrosamine |
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| Fan Peili,Li Zheng,Cao Jiaying,Xia Hansheng,Wang Wenping.Evaluation of Contrast-enhanced Ultrasound for the Rat Hepatocellular Carcinoma Model Induced by Diethylnitrosamine[J].Chinese Journal of Ultrasound in Medicine,2020(4):356-358. |
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| Authors: | Fan Peili Li Zheng Cao Jiaying Xia Hansheng Wang Wenping |
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| Institution: | (Department of Ultrasound,Zhongshan Hospital,Fudan University,Shanghai Institute of Ultrasound in Medicine and Engineering,Institute of Ultrasound Medicine and Engineering,Fudan University,Shanghai 200032,China;Academy of Clinical Medicine,Zhongshan Hospital,Fudan University,Shanghai 200032,China) |
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| Abstract: | Objective To explore the feasibility of contrast-enhanced ultrasound(CEUS)in evaluating a diethylnitrosamine(DEN)-induced hepatocellular carcinoma(HCC)rat model.Methods Twenty male Wistar rats were injected DEN at a dose of 30mg/kg body weight.The liver of 5rats underwent conventional ultrasound in turn at the 14th week.The rats underwent CEUS examination when the hepatic nodules were found by B-mode ultrasound.The hepatic nodules were confirmed histologically.Results The mortality of the experimental group was 45%(9/20)Out of the 20rats,16(80%)rats in the experimental group developed HCCs.Among all the HCCs,72.5%(29/40)were demonstrated on ultrasound.The major enhancement pattern of HCCs on CEUS was“hyper-enhancement without washout”(55.2%,16/29).Conclusions This animal model has a high tumor formation rate.CEUS can be applied to evaluate this animal model. |
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| Keywords: | Diethylnitrosamine Animals Hepatocellular carcinoma Contrast-enhanced ultrasound |
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