长链非编码RNA LINC00922促进胰腺导管腺癌的增殖、迁移和侵袭

目的 检测长链非编码RNA linc00922在胰腺癌组织及胰腺癌细胞系中表达,并探究linc00922对胰腺癌细胞系增殖、迁移和侵袭的影响。方法 利用illumina HiSeq X Ten高通量测序仪针对2015年-2016年间4对胰腺导管腺癌组织与癌旁组织进行高通量测序,建立lncRNA及mRNA表达谱,并挑选其中胰腺癌组织中显著高表达的lncRNA linc00922做为进一步研究对象。应用实时定量反转录聚合酶链反应(RT-qPCR)技术验证linc00922在10例胰腺癌及癌旁组织中表达情况。通过CCK-8增殖实验,划痕实验及Transwell实验探究linc00922对胰腺癌细胞系BXPC-3增殖、迁移及侵袭的调控作用。采用配对样本t检验,或独立样本t检验进行统计学分析。结果 前期通过高通量测序分析,发现胰腺癌组织中470个差异表达lncRNA,4373个差异表达mRNA,其中linc00922在胰腺癌组织中显著高表达。在10例胰腺癌组织及癌旁组织样本中,利用RT-qPCR进一步验证了linc00922在胰腺癌组织中显著高表达(p<0.05)。CCK-8增殖实验提示敲低linc00922表达的BXPC-3胰腺癌细胞增殖活力显著低于阴性对照组(p<0.05)。划痕实验提示敲低linc00922表达的BXPC-3胰腺癌细胞的迁移能力显著低于较阴性对照组(p<0.05)。Transwell侵袭实验提示敲低linc00922表达的BXPC-3胰腺癌细胞的侵袭能力显著低于较阴性对照组(p<0.05)。结论 linc00922在胰腺癌中显著高表达,并具有促进胰腺癌细胞增殖、迁移和侵袭的能力。

The long non-coding RNA LINC00922 promotes the proliferation, migration and invasion of pancreatic ductal adenocarcinoma cell line

Objective To evaluate the expression of long non-coding RNA linc00922 in pancreatic cancer tissues and pancreatic cancer cell lines and to explore its function on proliferation and invasion of pancreatic cancer cell line. Method Appling the illumina Hiseq X Ten to high-throughput sequence four pairs of pancreatic cancer tissues and corresponding adjacent normal tissues between 2015-2016. Establishing the lncRNA and mRNA expression profile with the sequencing results. Linc00922 was highly expressed in pancreatic cancer tissues and selected for further study. The expression of linc00922 was verified by the RT-qPCR with another 10 pairs of pancreatic tissues and adjacent normal tissues. To explore the function of linc00922 on proliferation and invasion of pancreatic cancer cell line by CCK-8 cell proliferation assay, wound-healing assay and transwell assay. Paired samples were analyzed by paired sample t test, or independent sample t test. Results Through high-throughput sequencing, 470 differentially expressed lncRNA and 4373 differentially expressed mRNA were discovered in pancreatic cancer tissues. The lncRNA linc00922 was over-expressed in pancreatic cancer. With another 10 paired pancreatic cancer tissues and adjacent normal tissues, the expression of linc00922 was verified by RT-qPCR. In addition, linc00922 was overexpressed in pancreatic cancer cell line BxPC-3. The CCK-8 cell proliferation assay showed that the proliferation activity of BxPC-3 was suppressed by down-expressing of linc00922(p<0.05). The wound healing assay showed that pancreatic cancer cells in linc00922 knocking-down group was significantly more slowly closing to the scratch area than the negative control group(p<0.05). The transwell assay showed that the number of migrating cells in linc00922 knocking-down group was significantly lower than the negative control group(p<0.05).