| 梅毒检测“灰区”样本的分析 |
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| 引用本文： | 曹树正,王文武,张真路.梅毒检测“灰区”样本的分析[J].实用医学杂志,2010,26(3). |
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| 作者姓名： | 曹树正 王文武 张真路 |
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| 作者单位： | 武汉亚洲心脏病医院检验中心,430022 |
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| 摘 要： | 目的:明确广东省中山市乙肝病毒感染者病毒基因型和基因亚型的分布状况,为临床抗病毒治疗方案的选择提供依据.方法:采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)法鉴定中山市127例乙肝病毒感染者的病毒基因型和基因亚型,结合血清学标志物进行分析.结果:经巢式PCR扩增HBV DNA阳性率92.1%(117/127);经酶切鉴定B基因型67例,占总感染者的52.7%,全部为Ba亚型,未发现Bj亚型;C基因型50例,占总感染者的39.4%,其中C1亚型41例.C2亚型9例;在HBeAg阳性感染者中B基因型占该群体的55.2%(48/87),而C基因型则占41.4%(36/87),C1亚型占34.5%(30/87),C2亚型占6.9%(6/87);在HBeAg阴性样品中以B基因型为主,占该群体的47.5%(19/40).而C基因型则占35.0%(14/40),C1亚型占27.5%(11/40),C2亚型占7.5%(3/40);不同E抗原状态其基因型分布有差异(χ2=7.460,P=0.024),而基因亚型分布则无差异(χ2=7.605,P=0.055).结论:中山市乙肝病毒感染者病毒基因型以Ba亚型为主,C基因型则以C1亚型为主.
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| 关 键 词： | 乙肝病毒 基因型 基因亚型 限制性片段长度多态性 抗病毒治疗 |
Analyses of syphilis assay for the samples in the gray zone |
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| CAO Shu-zheng,WANG Wen-wu,ZHANG Zhen-lu.Analyses of syphilis assay for the samples in the gray zone[J].The Journal of Practical Medicine,2010,26(3). |
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| Authors: | CAO Shu-zheng WANG Wen-wu ZHANG Zhen-lu |
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| Institution: | CAO Shu-zheng,WANG Wen-wu,ZHANG Zhen-lu.Department of Laboratory,Asia Heart Hospital,Wuhan 430022,China |
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| Abstract: | Objective To eliminate the omission and to get eligible syphilis test operating procedure through analysing the samples in the gray zone in ELISA test.Methods 13 802 serum samples were screened by TP-ELISA assay,and those in the gray zone were confirmed by toluidine red unheated-serum test(TRUST) and treponema pallidum particle agglutination test(TPPA) assay.Result There were 15 samples in the gray zone,among which 5 samples were confirmed positive by TPPA assay.Conclusion There is a little false negative r... |
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| Keywords: | Treponema Gray zone ELISA Toluidine red unheated-serum test Treponema pallidum particle agglutination test |
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