| HSV-1感染对神经胶质瘤细胞NGF和BDNF表达变化的研究 |
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| 引用本文： | 侯云,王斌,李玲,胡明,辛晓妮,钱冬萌,闫志勇,赵巍,宋旭霞.HSV-1感染对神经胶质瘤细胞NGF和BDNF表达变化的研究[J].实用医学杂志,2011,27(7):1132-1134. |
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| 作者姓名： | 侯云 王斌 李玲 胡明 辛晓妮 钱冬萌 闫志勇 赵巍 宋旭霞 |
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| 作者单位： | 青岛市医药生物技术重点实验室,青岛大学医学院,266071 |
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| 基金项目： | 国家自然科学基金项目，"泰山学者"建设工程专项经费资助项目 |
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| 摘 要： | 目的:探讨单纯疱疹病毒1型(HSV-1)感染时神经胶质细胞内源性神经生长因子(NGF)和脑源性神经生长因子(BDNF)的表达变化。方法:采用RT-PCR法检测HSV-1糖蛋白D(gD)基因扩增,RT-PCR法和Westernblotting法检测正常培养和感染HSV-1的神经胶质瘤(U251)细胞的NGF和BDNF。结果:HSV-1可感染U251细胞;正常U251细胞可表达NGF和BDNF;HSV-1感染U251细胞后,NGF和BDNF在感染后第6小时达高峰,之后随感染时间延长逐渐降低。结论:HSV-1可诱导U251细胞中的NGF和BDNF表达异常。
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| 关 键 词： | 神经胶质瘤 单纯疱疹病毒1型 神经生长因子 脑源性神经生长因子 |
Study of NGF and BDNF expression in HSV-1 infected human glioma cells |
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| HOU Yun,WANG Bin,LI Ling,HU Ming,XIN Xiao-ni,QIAN Dong-meng,YAN Zhi-yong,ZHAO Wei,SONG Xu-xia.Study of NGF and BDNF expression in HSV-1 infected human glioma cells[J].The Journal of Practical Medicine,2011,27(7):1132-1134. |
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| Authors: | HOU Yun WANG Bin LI Ling HU Ming XIN Xiao-ni QIAN Dong-meng YAN Zhi-yong ZHAO Wei SONG Xu-xia |
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| Institution: | HOU Yun,WANG Bin,LI Ling,HU Ming,XIN Xiao-ni,QIAN Dong-meng,YAN Zhi-yong,ZHAO Wei,SONG Xu-xia. Department of Microbiology,Key Laboratory of Medicine and Biotechnology of Qingdao,Qingdao University Medical College,Qingdao 266071,China |
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| Abstract: | Objective To investigate endogenic nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) expression in herpes simplex type-1-virus (HSV-1) -infected neuroglia cells. Methods U251 human glioma cells were cultured in RPMI-1640 medium and infected with HSV-1 in vitro to establish a cell model of viral infection. Establishment of HSV-1-infected U251 cells was identified by HSV-1 gD mRNA expression. NGF and BDNF expression was detected by RT-PCR and Western blotting, respectively, in normal U251... |
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| Keywords: | Glioma HSV-1 Nerve growth factor Brain-derived neurotrophic factor |
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