| 蛋白酶体活性抑制对大鼠海马tau蛋白的泛素化影响 |
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| 引用本文： | 刘英华,罗健东.蛋白酶体活性抑制对大鼠海马tau蛋白的泛素化影响[J].实用医学杂志,2011,27(3):386-389. |
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| 作者姓名： | 刘英华 罗健东 |
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| 作者单位： | 广州医学院药理教研室,510182 |
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| 基金项目： | 国家自然科学基金项目(编号:30901806); 广东省自然科学基金项目(编号:9451018201003650) |
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| 摘 要： | 目的:探讨蛋白酶体活性抑制对大鼠海马tau蛋白的泛素化影响.方法:利用双侧海马注射蛋白酶体的特异性抑制剂--乳胞素分别进行蛋白酶体活性检测、免疫印迹、免疫共沉淀,以检测大鼠海马内tau蛋白的泛素化改变情况.结果:(1)乳胞素引起蛋白酶体活性在24和48 h较对照组明显下降;(2)免疫共沉淀结果显示,蛋白酶体活性抑制使t...
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| 关 键 词： | 阿尔茨海默病 乳胞素 蛋白酶体 tau蛋白 泛素化 |
Effect of proteasome inhibition on the ubiquitination of tau protein |
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| LIU Ying-hua,LUO Jian-dong.Effect of proteasome inhibition on the ubiquitination of tau protein[J].The Journal of Practical Medicine,2011,27(3):386-389. |
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| Authors: | LIU Ying-hua LUO Jian-dong |
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| Institution: | LIU Ying-hua,LUO Jian-dong.Guangzhou Medical College,Guangzhou 510182,China |
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| Abstract: | Objective To investigate the effect of proteasome inhibition on the ubiquitination of tau protein.Methods We injected bilaterally lactacystin,a specific inhibitor of proteasome,into the hippocampus of the rats and employed proteasome activity assay,Western blotting and co-immunoprecipitation to observe the ubiquitination changes of tau protein in rats' hippocampus.Results We observed the following results in our experiment:(1) Lactacystin obviously inhibited the chymotrypsin-like activity of proteasome at t... |
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| Keywords: | Alzheimer disease Lactacystin Proteasome Tau protein Ubiquitination |
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