大蒜多糖预处理对缺氧缺糖/复氧复糖大鼠心肌细胞的拮抗作用

目的:研究大蒜多糖(GP)预处理对缺氧缺糖/复氧复糖损伤大鼠心肌细胞的拮抗作用研究,并初步探讨其作用机制。方法:采用连二亚硫酸钠(Na2S2O4)建立乳鼠心肌细胞糖氧剥离的A/R损伤模型,不同剂量GP(10、30和100mg/L)预处理24h后,比色法测定细胞培养液中iNOS和NO含量,测定细胞内SOD活性及MDA含量。结果:与A/R模型组比较,GP预处理可增加胞内SOD活性和培养液中iNOS活力,降低NO释放量和胞内MDA含量。结论:GP预处理对A/R损伤心肌细胞具有明显的抗氧化作用,可能与清除自由基的产生有关。

Inhibition of garlic polysaccharide pretreatment on cultured myocardiocytes injury induced by oxygen and glucose deprivation /reoxygenation

Objective To investigate the inhibition of garlic polysaccharide pretreatment on cultured myocardiocytes injury induced by oxygen and glucose deprivation /reoxygenation (A/R) and its mechanisms. Methods The model of oxygen and glucose deprivation/reoxygenation injury was developed by Na2S2O4 in cultured neonatal rat cardiomyocytes.The cultured cardiocytes were pretreated with different concentrations of garlic polysaccharide (10,30 and 100 mg·L-1)before anoxia/reoxygenation. The activities of SOD,iNOS,and the contents of MDA and NO were determined by colorimetry respectively. Results Compared with A/R model group, garlic polysaccharide could significantly increase the activity of SOD,inhibit the activities of iNOS in supernatant, decrease the contents of MDA and NO in a concentration dependent manner. Conclusion Garlic polysaccharide has a protective effect on myocardiocytes against anoxia/reoxygenation injury and the mechanism may be related with its oxygen free radical scavenging ability and regulation of the NO contents.