| 人脐带血间充质干细胞体外诱导分化为心肌细胞的实验研究 |
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| 引用本文： | 汤天军,杨波,杨汉东,张有顺,胡礼仪,李东锋,张继红.人脐带血间充质干细胞体外诱导分化为心肌细胞的实验研究[J].实用医学杂志,2005,21(21):2412-2415. |
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| 作者姓名： | 汤天军 杨波 杨汉东 张有顺 胡礼仪 李东锋 张继红 |
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| 作者单位： | 1. 430060,武汉大学人民医院心内科
2. 442008,湖北省十堰市,郧阳医学院附属东风医院心内科 |
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| 基金项目： | 本课题为湖北省教育厅科研基金项目（项目编号2002A03009） |
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| 摘 要： | 目的:研究人脐带血间充质干细胞体外诱导分化为心肌细胞的可行性。方法:由人脐静脉获得间充质干细胞,纯化培养后用不同浓度的5-氮杂胞苷(5-azacytidine)诱导,透射电镜、免疫组化及RT-PCR鉴定诱导后细胞。结果:经5-氮杂胞苷诱导后的脐带血间充质干细胞在透射电镜下观察到细胞内有明显的肌丝,横纹样结构形成,核呈卵圆形,位于细胞中央位置,胞质中可见富集的糖原颗粒及线粒体结构。免疫组化可见肌钙蛋白Ⅰ染色阳性。RT-PCR方法显示能表达缝隙连接膜通道蛋白Connexin-43基因。结论:人脐带血间充质干细胞可在体外经5-氮杂胞苷诱导分化为具有典型结构的心肌细胞。
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| 关 键 词： | 胎血 间质干细胞 细胞分化 心肌细胞 5-氮杂胞苷 |
| 收稿时间： | 2005-06-08 |
| 修稿时间： | 2005-06-082005-08-01 |
Differentiation of mesenchymal stem cells from human umbilical cord blood to cardiomyocytes with 5-azacytidine in vitro |
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| TANG Tian-jun,YANG Bo,YANG Han-dong,ZHANG You-shun,HU Li-yi,LI Dong-feng,ZHANG Ji-hong.Differentiation of mesenchymal stem cells from human umbilical cord blood to cardiomyocytes with 5-azacytidine in vitro[J].The Journal of Practical Medicine,2005,21(21):2412-2415. |
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| Authors: | TANG Tian-jun YANG Bo YANG Han-dong ZHANG You-shun HU Li-yi LI Dong-feng ZHANG Ji-hong |
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| Institution: | Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China |
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| Abstract: | Objective To explore the feasibility of mesenchymal stem cells(MSCs) from human umbilical cord blood (HUCB) differentiating to cardiomyocytes in vitro. Methods MSCs isolated from HUCB with Percoll density gradient solution were culture-expanded because the cells attached and grew on dishes, then MSCs were induced with different concentration of 5-azacytidine(5-Aza). The myotubes, cardiac specific antigen, gap junctions connexin-specific gene, cellular ultrastructure of differentiated cells were determined b... |
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| Keywords: | Fetal blood Mesenchymal stem cells Cell differrentiation Cardiomyocytes 5-Azacytidine |
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