前列腺癌浸润性树突状细胞CD1a、CD83的表达及与临床指标的相关性分析

目的探讨前列腺癌肿瘤浸润性树突状细胞（tumor infiltrating dendritic cells，TIDCs）的数量与活化功能的变化，并探讨分析前列腺癌组织TIDCs的表达与患者血清PSA值、年龄、Gleason评分、骨转移等情况的相关性。方法运用免疫组化Envision两步法染色技术标记28例前列腺癌组织及10例良性前列腺增生组织树突状细胞（dendriti ccell，DC）的CD1a及CD83分子，镜下观察被标记的DC的形态和分布情况并人工计数，比较良性和恶性前列腺组织的DC的CD1a及CD83的均数，使用student—t检验，P％0．05为差异有统计学意义。进行28例前列腺癌患者CD1a、CD83、年龄、PSA、Gleason评分的Pearson相关分析及Logistic回归分析，以．P〈0．05为相关性有统计学意义。结果前列腺癌组织DCCD1a多分布在癌周，染色浅，数量比前列腺增生组织CD1a的数量少，差异有统计学意义（P〈0．05）；前列腺癌组织DCCD83亦分布于癌周，染色较浅，数量比前列腺增生组织CD83的数量略多，差异无统计学意义（P〉0．05）；28例前列腺癌患者的CD1a、CD83、年龄、PSA的相关性不显著，相关无统计学意义（P〉0．05）。结论前列腺癌TIDCs多分布在癌周，总体数量（用CD1a标记）较良性前列腺组织少，活化的TIDCs（用CD83标记）的数量与良性组织相差不大；CD1a、CD83与年龄、PSA、Gleason评分及骨转移相关性不显著。

Investrgation of expression of CDla,CD83 molecules on tumor infiltrating dendritic cells in prostate cancer primary foci and clinical figures correlations

Objective To observe the quantity and activation of tumor infiltrating dendritic eells（TIDCs） in prostate cancer and investigate the correlation between the expression of TIDCs and clinical figures such as： serum PSA value, age, Gleason score and bone metastasis. Methods Marked CD1a and CD83 molecules on DC in 28 prostate cancer tissues and 10 benign prostatic hyperplasia tissues by using immunohistochemistry Envision two steps staining technique;and observed the differences of expression, form and distribution of DC between malignant prostate tissues and benign prostate tissues. Compared their quantity by student-t test,differences with statistical significanee：P〈0.05 ;then analyzed the correlation between the quantity of CD1a and CD83 and clinical outcomes such as：serum PSA value, age,Gleason score and bone metastasis by Pearson correlation analysis and logistic regression,marked correlation with statistical significance：P〈0.05. Results CD1a positive TIDCs in prostate cancer tissues distributed in peripheral mesenchymal tissue around foci,with shallow staining. The quantities of them were less than the ones in benign prostatic tissues. The difference had statistical significance （P〈0.05）. CD83 positive TIDCs in prostate cancer tissues also distributed mainly in peripheral mesenchymal tissue around foei,with shallow staining. The quantities of them were a little more than the ones in benign prostatic tissues. The difference had no statistical significance（P〉0.05）. There was not significant correlation between the quantity of CD1a and CD83 and clinical figures such as： serum PSA value, age, Gleason score and bone metastasis （P〉0.05）. Conclusions The holistic quantity of DC （CD1a positive） in prostate cancer tissues were less than the ones in benign prostate tissues;however,the quantity of activated DC in prostate cancer were no more than the one in benign prostate tissues. Significant correlation between the quantity of CD1a and CD83 and clinical outcomes such as：serum PSA value,age, Gleason score and bone metastasis were not found.