高压氧联合针刺对2型糖尿病大鼠认知功能的影响

目的:观察高压氧联合针刺治疗对2型糖尿病大鼠认知功能改善的影响作用。方法:将39只成年SD大鼠制备成2型糖尿病大鼠模型。予高糖高脂饮食6周后单次腹腔注射30mg/kg链脲佐菌素,注射3d后测得大鼠尾静脉随机血糖≥16.7mmol/L为模型制备成功。造模成功后7d采用Morris水迷宫测验检测所有糖尿病大鼠认知功能,并随机分为对照组、高压氧组和高压氧联合针刺组各13只,分组次日高压氧组和高压氧联合针刺组大鼠开始干预,1次/d,连续30d后再次采用Morris水迷宫测验检测所有大鼠认知功能。结果:干预30d后,与干预前及对照组比较,高压氧组及高压氧联合针刺组血糖均明显降低(P<0.05、0.01);高压氧联合针刺组血糖改善优于高压氧组(P<0.01)。与干预前及对照组比较,高压氧组及高压氧联合针刺组大鼠逃避潜伏期均明显缩短(P<0.01),穿越平台次数均明显增加(P<0.01);与高压氧组比较,高压氧联合针刺组逃避潜伏期缩短更显著(P<0.05),穿越平台次数增加更显著(P<0.01)。结论:高压氧治疗可明显改善2型糖尿病认知功能,高压氧联合针刺疗法对认知功能的改善作用更加显著,提示高压氧联合针刺疗法更有利于2型糖尿病患者认知功能障碍的早期康复。

Effects of hyperbaric oxygen combined with acupuncture on cognitive function in type 2 diabetic rats

Objective:To examine the effects of hyperbaric oxygen combined with acupuncture on cognitive function in type 2 diabetic rats.Methods:The type 2 diabetic rat models were established: 39 adult SD rats were fed on high-sugar and high-fat diet for 6 weeks, followed by intraperitoneally injection of streptozotocin(30 mg/kg) only once. Three days later, random blood glucose(RBG) was measured by tail vein sampling. If the RBG was more than 16.7 mmol/L, suggesting the successful establishment of the type 2 diabetic mellitus in rats. The Morris water maze test was used to measure the cognitive function in all diabetic rats. By using the random number table method the rats were divided into three groups: control group, hyperbaric oxygen group and hyperbaric oxygen combined with acupuncture group(n=13 in each group). The rats in hyperbaric oxygen group were treated with hyperbaric oxygen, and the rats in hyperbaric oxygen combined with acupuncture group were treated with hyperbaric oxygen combined with acupuncture(once a day, 30 days). After all the interventions, the cognitive function of all rats was tested by the Morris water maze test.Results:After 30 days of intervention, compared with the control group and before intervention, rats in the hyperbaric oxygen group and hyperbaric oxygen combined with acupuncture group had significantly lower blood glucose(P<0.05,0.01), and hyperbaric oxygen combined with acupuncture group was superior to hyperbaric oxygen group in blood glucose decrease(P<0.01). Compared with the control group and before intervention, rats in the hyperbaric oxygen group and hyperbaric oxygen combined with acupuncture group had shorter escape latency(P<0.01), more times of crossing the platform(P<0.01). Compared with hyperbaric oxygen group, rats in the hyperbaric oxygen combined with acupuncture group showed more significant improvement in escape latency(P<0.05) and numbers of crossing platforms(P<0.01).Conclusion: Hyperbaric oxygen therapy can significantly improve cognitive function of type 2 diabetes rats, and its combination with acupuncture has a more significant improvement effect. This may suggest that hyperbaric oxygen combined with acupuncture therapy is more conducive to early rehabilitation of cognitive impairment in type 2 diabetic patients.